# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4306860 | B | Inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate pretreated for 30 mins followed by substrate addition and measured after 30 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 27 | single protein format | Scientific Literature | ||
2. | ALA4306865 | A | Metabolic stability in human liver microsomes assessed as half life preincubated for 5 mins followed by NADPH addition measured upto 60 mins by LC-MS/MS analysis | Homo sapiens | 11 | microsome format | Scientific Literature | ||
3. | ALA4306866 | A | Apparent intrinsic clearance in human liver microsomes measured upto 60 mins by LC-MS/MS analysis | Homo sapiens | 11 | microsome format | Scientific Literature | ||
4. | ALA4306867 | A | Metabolic stability in mouse liver microsomes assessed as half life preincubated for 5 mins followed by NADPH addition measured upto 60 mins by LC-MS/MS analysis | Mus musculus | 11 | microsome format | Scientific Literature | ||
5. | ALA4306868 | A | Apparent intrinsic clearance in mouse liver microsomes measured upto 60 mins by LC-MS/MS analysis | Mus musculus | 11 | microsome format | Scientific Literature | ||
6. | ALA4306869 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate assessed as Kinact pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
7. | ALA4306870 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
8. | ALA4306871 | B | Irreversible inhibition of SMYD3 (unknown origin) using MAP3K2 peptide as substrate assessed as kinact/Ki ratio pretreated for 5 mins followed by substrate addition and measured after 60 mins by MTase Glo reagent based luminescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
9. | ALA4306872 | B | Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of cell proliferation incubated for 3 days by CellTiter-Glo luminescent assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
10. | ALA4306873 | B | Inhibition of SMYD3 in human HepG2 cells assessed as inhibition of anchorage-independent colony formation in 3D culture cells incubated for 1 to 2 weeks by thiazolyl blue tetrazolium bromide based | Homo sapiens | 7 | cell-based format | Scientific Literature |