#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4411043	F	Antiinvasive activity in mouse 4T1 cells assessed as inhibition of cell invasion at 0.2 mM incubated for 24 hrs by crystal violet staining based matrigel invasion assay relative to control	Mus musculus	3		cell-based format	Scientific Literature
2.	ALA4411044	T	Toxicity in BALB/c mouse xenografted with mouse 4T1 cells assessed as change in serum creatinine level at 10 mg/kg administered intratumorally and measured after 24 days	Mus musculus	4		organism-based format	Scientific Literature
3.	ALA4411045	T	Toxicity in BALB/c mouse xenografted with mouse 4T1 cells assessed as change in blood urea nitrogen level at 10 mg/kg administered intratumorally and measured after 24 days	Mus musculus	4		organism-based format	Scientific Literature
4.	ALA4411046	F	Antitumour activity against mouse 4T1 cells xenografted in BALB/c mouse assessed as increase in damage of tumor tissue at 10 mg/kg administered intratumorally and measured after 24 days by H and E staining based inverted optical microscopic method	Mus musculus	4		organism-based format	Scientific Literature
5.	ALA4411049	A	Stability in fetal bovine serum assessed as parent compound remaining measured after 12 hrs by RP-HPLC analysis	Bos taurus	3		cell-free format	Scientific Literature
6.	ALA4411050	F	Antiproliferative activity against mouse 4T1 cells at 0.1 mM incubated for 24 hrs by MTT assay	Mus musculus	4		cell-based format	Scientific Literature
7.	ALA4411051	F	Antiproliferative activity against mouse 4T1 cells assessed as cell death at 0.2 mM incubated for 24 hrs by MTT assay relative to control	Mus musculus	4		cell-based format	Scientific Literature
8.	ALA4411053	A	Hemolytic activity in BALB/c mouse RBC at 3.2 mM incubated for 2 hrs by UV-Vis spectrophotometric analysis	Mus musculus	5		cell-based format	Scientific Literature
9.	ALA4411055	F	Induction of apoptosis in mouse 4T1 cells at 0.8 mM incubated for 24 hrs by PI staining based inverted microscopic method	Mus musculus	3		cell-based format	Scientific Literature
10.	ALA4411057	F	Induction of cell cycle arrest in mouse 4T1 cells assessed as accumulation at G0/G1 phase at 0.4 mM incubated for 24 hrs by PI staining based flow cytometric analysis (Rvb = 37.78%)	Mus musculus	4		cell-based format	Scientific Literature
11.	ALA4411058	F	Induction of cell cycle arrest in mouse 4T1 cells assessed as accumulation at S phase at 0.4 mM incubated for 24 hrs by PI staining based flow cytometric analysis (Rvb =42.35%)	Mus musculus	4		cell-based format	Scientific Literature
12.	ALA4411059	F	Induction of cell cycle arrest in mouse 4T1 cells assessed as accumulation at G2/M phase at 0.4 mM incubated for 24 hrs by PI staining based flow cytometric analysis (Rvb = 19.82%)	Mus musculus	4		cell-based format	Scientific Literature
13.	ALA4411063	F	Induction of apoptosis in mouse 4T1 cells assessed as viable cells at 0.4 mM incubated for 24 hrs by Annexin V-FITC/PI based FACS analysis (Rvb = 92.2%)	Mus musculus	4		cell-based format	Scientific Literature
14.	ALA4411064	F	Induction of apoptosis in mouse 4T1 cells assessed as early apoptotic cells at 0.4 mM incubated for 24 hrs by Annexin V-FITC/PI based FACS analysis (Rvb = 0.76%)	Mus musculus	4		cell-based format	Scientific Literature
15.	ALA4411065	F	Induction of apoptosis in mouse 4T1 cells assessed as late apoptotic cells at 0.4 mM incubated for 24 hrs by Annexin V-FITC/PI based FACS analysis (Rvb = 6.62%)	Mus musculus	4		cell-based format	Scientific Literature
16.	ALA4411066	F	Induction of apoptosis in mouse 4T1 cells assessed as necrotic cells at 0.4 mM incubated for 24 hrs by Annexin V-FITC/PI based FACS analysis (Rvb = 0.47%)	Mus musculus	4		cell-based format	Scientific Literature
17.	ALA4411071	F	Inhibition of cell migration in mouse 4T1 cells assessed as wound closure rate at 0.2 mM measured up to 36 hrs by inverted optical microscopic method based wound healing assay (Rvb = 82%)	Mus musculus	4		cell-based format	Scientific Literature
18.	ALA4411073	F	Inhibition of cell migration in mouse 4T1 cells assessed as migration frequency at 0.2 mM incubated for 24 hrs by crystal violet staining based transwell assay relative to control	Mus musculus	4		cell-based format	Scientific Literature
19.	ALA4411079	F	Inhibition of metastasis in mouse 4T1 cells assessed as disruption in F-actin arrangement at 0.2 mM incubated for 24 hrs by Hoechst 33342 staining based confocal laser scanning microscopic method	Mus musculus	4		cell-based format	Scientific Literature
20.	ALA4411083	B	Inhibition of MMP9 in mouse 4T1 cells at 0.2 mM incubated for 24 hrs by coomassie brilliant blue staining based gelatin zymography assay relative to control	Mus musculus	3		cell-based format	Scientific Literature