# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4344313 | B | Inhibition of N-terminal His6-tagged human Mcl1 expressed in Escherichia coli Rosetta2 DE3 by fluorescent labeled Flu-Bid peptide based fluorescence polarization assay | Homo sapiens | 27 | ALA4342462 | assay format | Scientific Literature | |
2. | ALA4344314 | B | Inhibition of N-terminal His6-tagged human Bfl-1 (1 to 151 residues), expressed in Escherichia coli Rosetta2 DE3 by fluorescent labeled FAM-Bid peptide based fluorescence polarization assay | Homo sapiens | 27 | ALA4342462 | assay format | Scientific Literature | |
3. | ALA4344315 | B | Binding affinity to immobilized biotin-labeled and N-terminal His6-tagged human Mcl-1 expressed in Escherichia coli Rosetta2 DE3 by biolayer interferometry | Homo sapiens | 2 | ALA4342462 | assay format | Scientific Literature | |
4. | ALA4344316 | B | Binding affinity to immobilized biotin-labeled and N-terminal His6-tagged human Bfl-1 (1 to 151 residues), expressed in Escherichia coli Rosetta2 DE3 by biolayer interferometry | Homo sapiens | 2 | ALA4342462 | assay format | Scientific Literature | |
5. | ALA4344317 | B | Inhibition of N-terminal His6-tagged human Bcl-2 isoform 2 (1 to 34 residues and 92 to 202 residues with Bcl-xL sequence (35 to 50 residues) expressed in Escherichia coli Rosetta2 DE3 by fluorescent labeled FAM-Bid peptide based fluorescence polarization assay | Homo sapiens | 8 | ALA4342462 | assay format | Scientific Literature | |
6. | ALA4344318 | B | Inhibition of N-terminal His6-tagged human Bcl-xL (with internal deletion of 45 to 85 amino acids and C-terminal truncation for 212 to 233 residues) expressed in Escherichia coli Rosetta2 DE3 by fluorescent labeled FAM-Bid peptide based fluorescence polarization assay | Homo sapiens | 8 | ALA4342462 | assay format | Scientific Literature | |
7. | ALA4344324 | B | Inhibition of Mcl-1 in human M14 cells assessed as disruption of Mcl-1 and BL-Bim complexes formation at 100 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
8. | ALA4344325 | B | Inhibition of Bfl-1 in human M14 cells assessed as disruption of Bfl-1 and BL-Bim complexes formation at 100 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
9. | ALA4344326 | B | Inhibition of Mcl-1 in human M14 cells assessed as disruption of Mcl-1 and BL-Bim complexes formation by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
10. | ALA4344327 | B | Inhibition of Bfl-1 in human M14 cells assessed as disruption of Bfl-1 and BL-Bim complexes formation by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
11. | ALA4344328 | B | Inhibition of BCl2 in human M14 cells assessed as disruption of BCl2 and BL-Bim complexes formation at 50 to 100 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
12. | ALA4344329 | B | Inhibition of Bcl-xL in human M14 cells assessed as disruption of Bcl-xL and BL-Bim complexes formation at 50 to 100 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
13. | ALA4344333 | B | Inhibition of Mcl1 (unknown origin) expressed in Eu-Myc cells assessed as induction of cell death at 25 to 100 uM incubated for 24 hrs by violet LIVE/DEAD fixable dead cell staining based flow cytometry | Homo sapiens | 3 | ALA4342462 | cell-based format | Scientific Literature | |
14. | ALA4344334 | B | Inhibition of Bfl-1 (unknown origin) expressed in Eu-Myc cells assessed as induction of cell death at 25 to 100 uM incubated for 24 hrs by violet LIVE/DEAD fixable dead cell staining based flow cytometry | Homo sapiens | 3 | ALA4342462 | cell-based format | Scientific Literature | |
15. | ALA4344335 | B | Inhibition of Mcl-1 in human M14 cells assessed as disruption of Mcl-1 and BL-Bim complexes formation at 50 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 1 | ALA4342462 | cell-based format | Scientific Literature | |
16. | ALA4344336 | B | Inhibition of Bfl-1 in human M14 cells assessed as disruption of Bfl-1 and BL-Bim complexes formation at 50 uM by biotin-labeled Bim peptide based biotin-streptavidin pull down assay | Homo sapiens | 1 | ALA4342462 | cell-based format | Scientific Literature | |
17. | ALA4344338 | B | Inhibition of Bcl2 (unknown origin) expressed in Eu-Myc cells assessed as induction of cell death at 25 to 100 uM incubated for 24 hrs by violet LIVE/DEAD fixable dead cell staining based flow cytometry | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
18. | ALA4344339 | B | Inhibition of Bcl-xL (unknown origin) expressed in Eu-Myc cells assessed as induction of cell death at 25 to 100 uM incubated for 24 hrs by violet LIVE/DEAD fixable dead cell staining based flow cytometry | Homo sapiens | 2 | ALA4342462 | cell-based format | Scientific Literature | |
19. | ALA5163773 | F | Growth inhibition of human MM1.S cells incubated for 4 days by WST assay | Homo sapiens | 3 | ALA5154839 | cell-based format | Scientific Literature | |
20. | ALA5163776 | B | Displacement of fluorescent-labeled BID-BH3 peptide from His-tagged human BFL-1 (1 to 151 residues) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant by fluorescence polarization-based competitive binding assay | Homo sapiens | 3 | ALA5154839 | assay format | Scientific Literature |