| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4326666 | F | Antiinflammatory activity in ICR mouse primary peritoneal macrophages assessed as inhibition of LPS-induced IL-6 production at 20 uM preincubated for 60 mins followed by LPS stimulation and measured after 24 hrs by ELISA relative to control | Mus musculus | 48 | ALA4325844 | cell-based format | Scientific Literature | |
| 2. | ALA4326667 | F | Antiinflammatory activity in ICR mouse primary peritoneal macrophages assessed as inhibition of LPS-induced TNFalpha production at 20 uM preincubated for 60 mins followed by LPS stimulation and measured after 24 hrs by ELISA relative to control | Mus musculus | 48 | ALA4325844 | cell-based format | Scientific Literature | |
| 3. | ALA4326668 | F | Antiinflammatory activity in ICR mouse primary peritoneal macrophages assessed as inhibition of LPS-induced IL-6 production preincubated for 2 hrs followed by LPS stimulation and measured after 22 hrs by ELISA | Mus musculus | 4 | ALA4325844 | cell-based format | Scientific Literature | |
| 4. | ALA4326669 | F | Antiinflammatory activity in ICR mouse primary peritoneal macrophages assessed as inhibition of LPS-induced TNFalpha production preincubated for 2 hrs followed by LPS stimulation and measured after 22 hrs by ELISA | Mus musculus | 4 | ALA4325844 | cell-based format | Scientific Literature | |
| 5. | ALA4326670 | F | Potency index, ratio of TJ-M2010-5 IC50 to test compound IC50 for inhibition of LPS-induced TNFalpha production in ICR mouse primary peritoneal macrophages | Mus musculus | 1 | ALA4325844 | cell-based format | Scientific Literature | |
| 6. | ALA4326671 | A | Cytotoxicity against ICR mouse primary peritoneal macrophages assessed as cell viability at 20 uM after 24 hrs by MTT assay relative to control | Mus musculus | 3 | ALA4325844 | cell-based format | Scientific Literature | |
| 7. | ALA4326672 | A | Cytotoxicity against rat NRK cells assessed as cell viability at 20 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 3 | ALA4325844 | cell-based format | Scientific Literature | |
| 8. | ALA4326673 | A | Cytotoxicity against rat H9c2 cells assessed as cell viability at 20 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 3 | ALA4325844 | cell-based format | Scientific Literature | |
| 9. | ALA4326674 | B | Inhibition of LPS-induced HA-tagged MyD88 (unknown origin) interaction with FLAG-tagged MyD88 (unknown origin) expressed in HEK293 cells at 20 uM after 48 hrs by co-immunoprecipitation method | Homo sapiens | 2 | ALA4325844 | cell-based format | Scientific Literature | |
| 10. | ALA4326675 | B | Inhibition of TLR4/MD2 interaction in LPS-induced mouse RAW264.7 at 20 uM measured after 30 mins by co-immunoprecipitation method | Mus musculus | 1 | ALA4325844 | cell-based format | Scientific Literature | |
| 11. | ALA4326676 | B | Inhibition of LPS-induced HA/FLAG-tagged TLR4 (unknown origin) interaction with MD2 (unknown origin) expressed in HEK293 cells at 20 uM after 48 hrs by co-immunoprecipitation method | Homo sapiens | 1 | ALA4325844 | cell-based format | Scientific Literature | |
| 12. | ALA4326677 | B | Binding affinity to recombinant human MyD88 TIR domain (157 to 296 residues) expressed in Escherichia coli BL21(DE3) by surface plasmon resonance analysis | Homo sapiens | 2 | ALA4325844 | assay format | Scientific Literature | |
| 13. | ALA4326678 | B | Potency index, ratio of TJ-M2010-5 Kd to test compound Kd for recombinant human MyD88 TIR domain (157 to 296 residues) expressed in Escherichia coli BL21(DE3) | Homo sapiens | 1 | ALA4325844 | assay format | Scientific Literature | |
| 14. | ALA4326679 | B | Binding affinity to recombinant human 10His-tagged TLR4 (24 to 631 residues) by SPR assay | Homo sapiens | 1 | ALA4325844 | single protein format | Scientific Literature | |
| 15. | ALA4326680 | B | Inhibition of MyD88 in ICR mouse primary peritoneal macrophages assessed as reduction in LPS-induced JNK phosphorylation at 20 uM preincubated for 30 mins followed by LPS-stimulation and measured after 20 mins by Western blot analysis | Mus musculus | 2 | ALA4325844 | single protein format | Scientific Literature | |
| 16. | ALA4326681 | B | Inhibition of MyD88 in ICR mouse primary peritoneal macrophages assessed as reduction in LPS-induced ERK phosphorylation at 20 uM preincubated for 30 mins followed by LPS-stimulation and measured after 20 mins by Western blot analysis | Mus musculus | 2 | ALA4325844 | single protein format | Scientific Literature | |
| 17. | ALA4326682 | B | Inhibition of MyD88 in ICR mouse primary peritoneal macrophages assessed as reduction in LPS-induced p38 MAPK phosphorylation at 20 uM preincubated for 30 mins followed by LPS-stimulation and measured after 20 mins by Western blot analysis | Mus musculus | 2 | ALA4325844 | single protein format | Scientific Literature | |
| 18. | ALA4326683 | B | Inhibition of MyD88 in ICR mouse primary peritoneal macrophages assessed as reduction in LPS-induced IkappaB levels at 20 uM preincubated for 30 mins followed by LPS-stimulation and measured after 20 mins by Western blot analysis | Mus musculus | 1 | ALA4325844 | single protein format | Scientific Literature | |
| 19. | ALA4326684 | B | Inhibition of MyD88 in ICR mouse primary peritoneal macrophages assessed as reduction in LPS-induced NFkappaB translocation at 20 uM preincubated for 30 mins followed by LPS-stimulation and measured after 20 mins by immunofluorescence microscopic analysis | Mus musculus | 1 | ALA4325844 | single protein format | Scientific Literature | |
| 20. | ALA4326685 | B | Inhibition of MyD88 in mouse RAW264.7 cells expressing EGFP-fused NFkappaB at 10 uM preincubated for 30 mins followed by LPS-stimulation and measured after 1 hr by fluorescence assay | Mus musculus | 1 | ALA4325844 | cell-based format | Scientific Literature |
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