# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4349912 | B | Inverse agonist activity at GST-tagged RORgammat LBD (unknown origin) using fluorescein-D22 co-activator peptide as substrate incubated for 1 hr by TR-FRET assay | Homo sapiens | 46 | ALA4346735 | single protein format | Scientific Literature | |
2. | ALA4349913 | B | Transactivation of recombinant GAL4-DBD fused RORgammat LBD (unknown origin) expressed in HEK293T cells incubated for 18 to 24 hrs by dual-Glo luciferase assay | Homo sapiens | 44 | ALA4346735 | cell-based format | Scientific Literature | |
3. | ALA4349921 | A | Inhibition of CYP1A2 in human liver microsomes using phenacetin as substrate preincubated for 5 mins followed by NADPH addition measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
4. | ALA4349922 | A | Inhibition of CYP2C9 in human liver microsomes using diclofenac as substrate preincubated for 5 mins followed by NADPH addition measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
5. | ALA4349923 | A | Inhibition of CYP2C19 in human liver microsomes using (S)-mephenytoin as substrate preincubated for 5 mins followed by NADPH addition measured after 40 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
6. | ALA4349924 | A | Inhibition of CYP2D6 in human liver microsomes using dextromethorphan as substrate preincubated for 5 mins followed by NADPH addition measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
7. | ALA4349925 | A | Inhibition of CYP3A4 in human liver microsomes using midazolam as substrate preincubated for 5 mins followed by NADPH addition measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
8. | ALA4349926 | A | Inhibition of CYP3A4 in human liver microsomes using testosterone as substrate preincubated for 5 mins followed by NADPH addition measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 44 | ALA4346735 | microsome format | Scientific Literature | |
9. | ALA4349927 | P | Solubility of the compound at pH 2.1 | 44 | ALA4346735 | small-molecule physicochemical format | Scientific Literature | ||
10. | ALA4349928 | P | Solubility of the compound at pH 4 | 44 | ALA4346735 | small-molecule physicochemical format | Scientific Literature | ||
11. | ALA4349929 | P | Solubility of the compound at pH 7.4 | 44 | ALA4346735 | small-molecule physicochemical format | Scientific Literature | ||
12. | ALA4349930 | A | Cytotoxicity against Balb/c mouse splenocytes assessed as cell viability in presence of anti-CD3/anti-CD28 measured after 24 hrs | Mus musculus | 1 | ALA4346735 | cell-based format | Scientific Literature | |
13. | ALA4349931 | B | Transrepression of RORgammat in Balb/c mouse splenocytes assessed as reduction in anti-CD3/anti-CD28 stimulated IL-17 level measured after 48 hrs in presence of anti-CD3/anti-CD28 by ELISA | Mus musculus | 1 | ALA4346735 | cell-based format | Scientific Literature | |
14. | ALA4349932 | B | Transrepression of RORgammat in Balb/c mouse splenocytes assessed as inhibition of anti-CD3/anti-CD28 stimulated IL-17 gene expression at 0.3 uM measured after 24 hrs in presence of anti-CD3/anti-CD28 | Mus musculus | 4 | ALA4346735 | cell-based format | Scientific Literature | |
15. | ALA4349933 | B | Transrepression of RORgammat in Balb/c mouse splenocytes assessed as inhibition of anti-CD3/anti-CD28 stimulated IL-17 gene expression at 3 uM measured after 24 hrs in presence of anti-CD3/anti-CD28 | Mus musculus | 4 | ALA4346735 | cell-based format | Scientific Literature | |
16. | ALA4349934 | B | Transrepression of RORgammat in Balb/c mouse splenocytes assessed as inhibition of anti-CD3/anti-CD28 stimulated IL-17 gene expression at 30 uM measured after 24 hrs in presence of anti-CD3/anti-CD28 | Mus musculus | 4 | ALA4346735 | cell-based format | Scientific Literature | |
17. | ALA4349935 | B | Transrepression of RORgammat in cytostim stimulated human whole blood assessed as suppression of IL-17 secretion incubated for 24 hrs | Homo sapiens | 1 | ALA4346735 | tissue-based format | Scientific Literature | |
18. | ALA4349936 | B | Inverse agonist activity at RORgammat in mouse splenocytes derived naive T cells assessed as suppression of T cell differentiation to Th17 cells by measuring IL-17 secretion in presence of anti-CD4/anti-IFNgamma/TGFbeta/IL-6 for 6 days by ELISA | Mus musculus | 4 | ALA4346735 | cell-based format | Scientific Literature | |
19. | ALA4349937 | A | Cytotoxicity against mouse splenocytes assessed as cell viability incubated for 6 days in presence of anti-CD4/anti-IFNgamma/TGFbeta/IL-6 | Mus musculus | 1 | ALA4346735 | cell-based format | Scientific Literature | |
20. | ALA4349938 | A | Metabolic stability in mouse hepatocytes assessed as parent compound remaining after 60 mins | Mus musculus | 1 | ALA4346735 | cell-based format | Scientific Literature |