| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4416026 | B | Inhibition of N-terminal 6x-His-tagged recombinant human cIAP2-BIR3 (244 to 349 residues) expressed in Escherichia coli incubated for 2 hrs without 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 2. | ALA4416027 | B | Inhibition of N-terminal His-tagged human XIAP-BIR3 (253 to 347 residues) expressed in Escherichia coli BL21-Gold(DE3) pLysS incubated for 2 hrs without 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 3. | ALA4416028 | B | Inhibition of N-terminal His-tagged human XIAP-BIR3 (253 to 347 residues) expressed in Escherichia coli BL21-Gold(DE3) pLysS incubated for 2 hrs after 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 4. | ALA4416029 | B | Binding affinity to N-terminal His-tagged human XIAP-BIR3 (253 to 347 residues) expressed in Escherichia coli BL21-Gold(DE3) pLysS assessed as change in melting temperature incubated for for 2 hrs at room temperature by SYPRO orange dye based thermal shift assay | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 5. | ALA4416030 | B | Binding affinity to N-terminal His-tagged human XIAP-BIR3 (253 to 347 residues) expressed in Escherichia coli BL21-Gold(DE3) pLysS assessed as change in melting temperature incubated for for 6 hrs at 37 degC by SYPRO orange dye based thermal shift assay | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 6. | ALA4416031 | B | Inhibition of N-terminal 6x-His-tagged recombinant human cIAP1-BIR3 (258 to 363 residues) expressed in Escherichia coli incubated for 2 hrs without 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 7. | ALA4416032 | B | Inhibition of N-terminal 6x-His-tagged recombinant human cIAP1-BIR3 (258 to 363 residues) expressed in Escherichia coli incubated for 2 hrs after 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 8. | ALA4416033 | B | Inhibition of N-terminal 6x-His-tagged recombinant human cIAP2-BIR3 (244 to 349 residues) expressed in Escherichia coli incubated for 2 hrs after 6 hrs pre-incubation with enzyme by biotinylated AVPI peptide based DELFIA | Homo sapiens | 5 | ALA4414592 | assay format | Scientific Literature | |
| 9. | ALA4416034 | P | Stability in aqueous buffer at 100 uM to 2 mM by 1H 1D NMR spectroscopy | 1 | ALA4414592 | small-molecule physicochemical format | Scientific Literature | ||
| 10. | ALA4416035 | P | Solubility in pH 7.5 aqueous buffer at 25 degC by 1H 1D NMR spectroscopy | 2 | ALA4414592 | small-molecule physicochemical format | Scientific Literature | ||
| 11. | ALA4416036 | A | Stability in mouse plasma assessed as half life at 200 uM at 37 degC incubated up to 120 mins by mass spectrometry | Mus musculus | 4 | ALA4414592 | cell-free format | Scientific Literature | |
| 12. | ALA4416037 | B | Covalent binding affinity to HA-BIR3 domain of XIAP (unknown origin) expressed in HEK293T cells assessed as increase in band intensity at 10 uM incubated for 6 hrs by Western blot analysis | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 13. | ALA4416038 | B | Induction of cIAP1 degradation in human SK-MEL-28 cells assessed as reduction in cIAP1 protein level at 1 uM incubated for 3 hrs by Western blot analysis | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 14. | ALA4416039 | B | Induction of cIAP1 degradation in human A549 cells assessed as reduction in cIAP1 protein level at 1 uM incubated for 3 hrs by Western blot analysis | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 15. | ALA4416040 | B | Induction of cIAP2 degradation in human SK-MEL-28 cells assessed as reduction in cIAP2 protein level at 1 uM incubated for 3 hrs by Western blot analysis | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 16. | ALA4416041 | B | Induction of cIAP2 degradation in human A549 cells assessed as reduction in cIAP2 protein level at 1 uM incubated for 3 hrs by Western blot analysis | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 17. | ALA4416042 | F | Induction of apoptosis in human SK-MEL-28 cells assessed as increase in caspase-3/7 activity at 2.5 uM incubated for 36 hrs by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 18. | ALA4416043 | F | Induction of apoptosis in human A549 cells assessed as increase in caspase-3/7 activity at 2.5 uM incubated for 36 hrs by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 19. | ALA4416044 | F | Induction of apoptosis in human SK-MEL-28 cells assessed as increase in caspase-3/7 activity at 2.5 uM incubated for 36 hrs in presence of 1 ng/mL TNFlpha by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature | |
| 20. | ALA4416045 | F | Induction of apoptosis in human A549 cells assessed as increase in caspase-3/7 activity at 2.5 uM incubated for 36 hrs in presence of 1 ng/mL TNFlpha by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 5 | ALA4414592 | cell-based format | Scientific Literature |
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