# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4380027 | B | Inhibition of ALR2 in rat lens assessed as reduction in sorbitol accumulation at 50 uM preincubated for 30 mins followed by D-glucose addition and measured after 3 hrs by resazurin based fluorescence analysis relative to control | Rattus norvegicus | 3 | ALA4376851 | assay format | Scientific Literature | |
2. | ALA4380028 | B | Inhibition of rat lens ALR2 using D,L-glyceraldehyde as substrate incubated for 1 min and measured up to 4 mins using 1% DMSO-dissolved compound by spectrophotometric analysis | Rattus norvegicus | 5 | ALA4376851 | single protein format | Scientific Literature | |
3. | ALA4380029 | B | Inhibition of rat lens ALR2 using D,L-glyceraldehyde as substrate incubated for 1 min and measured up to 4 mins using H2O-dissolved compound by spectrophotometric analysis | Rattus norvegicus | 5 | ALA4376851 | single protein format | Scientific Literature | |
4. | ALA4380030 | B | Inhibition of rat kidney ALR1 using sodium D-glucuronate as substrate incubated for 1 min and measured up to 4 mins using H2O-dissolved compound by spectrophotometric analysis | Rattus norvegicus | 5 | ALA4376851 | assay format | Scientific Literature | |
5. | ALA4380031 | B | Inhibition of rat kidney ALR1 at 100 uM using sodium D-glucuronate as substrate incubated for 1 min and measured up to 4 mins using H2O-dissolved compound by spectrophotometric analysis relative to control | Rattus norvegicus | 5 | ALA4376851 | assay format | Scientific Literature | |
6. | ALA4380032 | B | Selectivity index, ratio of IC50 for rat kidney ALR1 using sodium D-glucuronate as substrate incubated for 1 min and measured up to 4 mins using H2O-dissolved compound to IC50 for rat lens ALR2 using D,L-glyceraldehyde as substrate incubated for 1 min and measured up to 4 mins using H2O-dissolved compound | Rattus norvegicus | 5 | ALA4376851 | assay format | Scientific Literature | |
7. | ALA4380033 | B | Inhibition of human AKR1B1 expressed in Escherichia coli using D,L-glyceraldehyde as substrate incubated for 1 min and measured up to 4 mins using by spectrophotometric analysis | Homo sapiens | 2 | ALA4376851 | assay format | Scientific Literature | |
8. | ALA4380034 | B | Inhibition of ALR2 in rat lens assessed as reduction in sorbitol accumulation preincubated for 30 mins followed by D-glucose addition and measured after 3 hrs by resazurin based fluorescence analysis | Rattus norvegicus | 1 | ALA4376851 | assay format | Scientific Literature | |
9. | ALA4380035 | B | Inhibition of human AKR1B10 using D,L-glyceraldehyde as substrate incubated for 1 min and measured up to 4 mins using by spectrophotometric analysis | Homo sapiens | 1 | ALA4376851 | single protein format | Scientific Literature | |
10. | ALA4380036 | B | Inhibition of rat lens ALR2 using methylglyoxal as substrate incubated for 1 min and measured up to 4 mins by spectrophotometric analysis | Rattus norvegicus | 2 | ALA4376851 | single protein format | Scientific Literature | |
11. | ALA4380037 | B | Inhibition of rat lens ALR2 using 4-hydroxynonenal as substrate incubated for 1 min and measured up to 4 mins by spectrophotometric analysis | Rattus norvegicus | 2 | ALA4376851 | single protein format | Scientific Literature | |
12. | ALA4380038 | B | Inhibition of rat lens ALR2 using 4-hydroxynonenal glutathione as substrate incubated for 1 min and measured up to 4 mins by spectrophotometric analysis | Rattus norvegicus | 2 | ALA4376851 | single protein format | Scientific Literature | |
13. | ALA4380039 | B | Inhibition of sheep liver sorbitol dehydrogenase at 100 uM using sorbitol as substrate measured for 4 mins in presence of NAD+ by spectrophotometric analysis relative to control | Ovis aries | 1 | ALA4376851 | assay format | Scientific Literature | |
14. | ALA4380040 | F | Antioxidant activity assessed as DPPH radical scavenging activity by measuring reduction in absorbance at 200 uM after 30 mins (Rvb = 0.013 +/- 0.012 No_unit) | 4 | ALA4376851 | assay format | Scientific Literature | ||
15. | ALA4380041 | B | Selectivity index, ratio of IC50 for human AKR1B10 to IC50 for human AKR1B1 expressed in Escherichia coli | Homo sapiens | 1 | ALA4376851 | assay format | Scientific Literature |