# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4397084 | A | Activation of non-specific gene expression in HEK293- FlpIn-FM cells harboring LTR driven CBG reporter co-expressing CMV driven CBR reporter assessed as CMV driven gene expression at 40 uM incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay | Homo sapiens | 6 | ALA4396867 | cell-based format | Scientific Literature | |
2. | ALA4397085 | A | Activation of non-specific gene expression in HEK293- FlpIn-FM cells harboring LTR driven CBR reporter co-expressing CMV driven CBG reporter assessed as CMV driven gene expression incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay | Homo sapiens | 102 | ALA4396867 | cell-based format | Scientific Literature | |
3. | ALA4397086 | B | Activation of Tat-mediated HIV1 transcription in HEK293- FlpIn-FM cells harboring LTR driven CBR reporter co-expressing CMV driven CBG reporter assessed as LTR-driven gene expression incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay | Human immunodeficiency virus 1 | 102 | ALA4396867 | cell-based format | Scientific Literature | |
4. | ALA4397087 | B | Activation of Tat-mediated HIV1 transcription in HEK293-FlpIn.RV cells harboring LTR driven CBG reporter co-expressing CMV driven CBR reporter assessed as LTR-driven gene expression incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay | Human immunodeficiency virus 1 | 1 | ALA4396867 | cell-based format | Scientific Literature | |
5. | ALA4397088 | A | Activation of non-specific gene expression in HEK293-FlpIn.RV cells harboring LTR driven CBR reporter co-expressing CMV driven CBG reporter assessed as CMV driven gene expression incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay | Homo sapiens | 1 | ALA4396867 | cell-based format | Scientific Literature | |
6. | ALA4397089 | P | Aqueous solubility of the compound in 0.01M HCl at pH 2 by nephelometry | 13 | ALA4396867 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA4397090 | P | Aqueous solubility of the compound in phosphate buffer at pH 6.5 by nephelometry | 13 | ALA4396867 | small-molecule physicochemical format | Scientific Literature | ||
8. | ALA4397091 | A | Half-life in human liver microsomes at 1 uM in presence of NADPH regenerating system by LC/MS analysis | Homo sapiens | 13 | ALA4396867 | microsome format | Scientific Literature | |
9. | ALA4397092 | A | Intrinsic clearance in human liver microsomes assessed per mg protein at 1 uM in presence of NADPH regenerating system by LC/MS analysis | Homo sapiens | 13 | ALA4396867 | microsome format | Scientific Literature | |
10. | ALA4397093 | A | Half-life in mouse liver microsomes at 1 uM in presence of NADPH regenerating system by LC/MS analysis | Mus musculus | 13 | ALA4396867 | microsome format | Scientific Literature | |
11. | ALA4397094 | A | Intrinsic clearance in mouse liver microsomes assessed per mg protein at 1 uM in presence of NADPH regenerating system by LC/MS analysis | Mus musculus | 13 | ALA4396867 | microsome format | Scientific Literature | |
12. | ALA4397095 | A | Cytotoxicity against human HepG2 cells assessed as reduction in cell growth incubated for 48 hrs by CellTiter-Glo assay | Homo sapiens | 14 | ALA4396867 | cell-based format | Scientific Literature | |
13. | ALA4397096 | A | Cytotoxicity against HEK293 cells assessed as reduction in cell growth incubated for 48 hrs by MTS assay | Homo sapiens | 14 | ALA4396867 | cell-based format | Scientific Literature | |
14. | ALA4397097 | B | Activation of Tat-mediated HIV1 transcription in HEK293- FlpIn-FM cells harboring LTR driven CBR reporter co-expressing CMV driven CBG reporter assessed as maximum LTR activity at 20 uM incubated for 48 hr using Chroma-Glo substrate by luciferase dual reporter cellular assay relative to control | Human immunodeficiency virus 1 | 2 | ALA4396867 | cell-based format | Scientific Literature | |
15. | ALA4397105 | B | Activation of Tat-mediated HIV1 transcription in J-Lat 10.6 cells harboring LTR driven GFP reporter co-expressing CMV driven RFP reporter assessed as LTR-driven gene expression incubated for 48 hr by FACSCalibur flow cytometry | Human immunodeficiency virus 1 | 14 | ALA4396867 | cell-based format | Scientific Literature | |
16. | ALA4397106 | A | Activation of non-specific gene expression in human J-Lat 10.6 cells harboring LTR driven GFP reporter co-expressing CMV driven RFP reporter assessed as CMV driven gene expression incubated for 48 hr by FACSCalibur flow cytometry | Homo sapiens | 14 | ALA4396867 | cell-based format | Scientific Literature | |
17. | ALA4397107 | B | Inhibition of His-tagged BRD4 bromodomain 1 (unknown origin) using H4 peptide as substrate pre-incubated for 30 mins followed by substrate addition and measured after 1 hr by alpha screen assay relative to control | Homo sapiens | 1 | ALA4396867 | single protein format | Scientific Literature | |
18. | ALA4397108 | B | Inhibition of His-tagged BRD4 bromodomain 2 (unknown origin) using H4 peptide as substrate pre-incubated for 30 mins followed by substrate addition and measured after 1 hr by alpha screen assay relative to control | Homo sapiens | 1 | ALA4396867 | single protein format | Scientific Literature | |
19. | ALA4397125 | B | Synergistic activation of Tat-mediated HIV1 transcription in HEK293- FlpIn-FM cells at 10 uM incubated for 48 hrs in presence of Ricolinostat | Human immunodeficiency virus 1 | 2 | ALA4396867 | cell-based format | Scientific Literature | |
20. | ALA4397126 | B | Synergistic activation of Tat-mediated HIV1 transcription in J-Lat 10.6 cells at 10 uM incubated for 48 hrs in presence of Ricolinostat | Human immunodeficiency virus 1 | 2 | ALA4396867 | cell-based format | Scientific Literature |