#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4364954	Functional	Antiviral activity against pseudotyped Enterovirus A71 infected in human muscular rhabdomyosarcoma cells treated prior to viral infection and measured after 24 hrs by luminescence method	Enterovirus A71	32	ALA4364253	organism-based format	Scientific Literature
2.	ALA4364955	ADME	Cytotoxicity against human RD cells at 100 uM after 24 hrs by WST-1 assay relative to control	Homo sapiens	32	ALA4364253	cell-based format	Scientific Literature
3.	ALA4364957	Binding	Reversible inhibition of Enterovirus 71 3C protease assessed as overall inhibition constant using NMA-IEALFQGPPK(DNP)FR as substrate pre-incubated for 30 mins followed by substrate addition and measured over 30 mins by fluorescence method	Enterovirus A71	28	ALA4364253	assay format	Scientific Literature
4.	ALA4364959	Binding	Binding affinity to Enterovirus 71 3C protease assessed as enzyme-compound covalent adduct formation via Cys147 residue after 1 hr by HPLC-ESI/MS analysis	Enterovirus A71	2	ALA4364253	assay format	Scientific Literature
5.	ALA4364961	Binding	Inhibition of Enterovirus 71 3C protease assessed as induction of rapid equilibrium for enzyme-inhibitor complex formation up to 1.5 uM using NMA-IEALFQGPPK(DNP)FR as substrate by fluorescence method	Enterovirus A71	1	ALA4364253	assay format	Scientific Literature
6.	ALA4364962	Binding	Reversible inhibition of Enterovirus 71 3C protease assessed as recovery of enzyme activity at 10 to 20 equivalent using peptide substrate pre-incubated for 1 hr followed by dilution prior to substrate addition using ultra-filtered sample relative to control	Enterovirus A71	6	ALA4364253	assay format	Scientific Literature