| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4417100 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and [14C] IPP as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by scintillation counter method | Homo sapiens | 3 | ALA4414654 | assay format | Scientific Literature | |
| 2. | ALA4417104 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as release of PPi using GPP and IPP as substrate preincubated for 30 mins in absence of catalase followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 2 | ALA4414654 | assay format | Scientific Literature | |
| 3. | ALA4417105 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as release of PPi using GPP and IPP as substrate preincubated for 30 mins in presence of catalase followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 2 | ALA4414654 | assay format | Scientific Literature | |
| 4. | ALA4417106 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged GGPPS expressed in Escherichia coli BL21 (DE3) using FPP and [14C] IPP as substrate preincubated for 15 mins with FPP followed by [14C] IPP addition and further incubated for 20 mins by scintillation counting method | Homo sapiens | 57 | ALA4414654 | assay format | Scientific Literature | |
| 5. | ALA4417107 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 58 | ALA4414654 | assay format | Scientific Literature | |
| 6. | ALA4417108 | B | Selectivity index, ratio of IC50 for human N-terminal TEV protease cleavage site-fused-His6-tagged GGPPS expressed in Escherichia coli BL21 (DE3) to IC50 for human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 16 | ALA4414654 | assay format | Scientific Literature | |
| 7. | ALA4417109 | B | Ratio of IC50 for Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and [14C] IPP as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by scintillation counter method to IC50 for Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as release of PPi using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 3 | ALA4414654 | assay format | Scientific Literature | |
| 8. | ALA4417110 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition in presence of 0.01 % Triton X-100 by continuous spectrophotometric assay | Homo sapiens | 14 | ALA4414654 | assay format | Scientific Literature | |
| 9. | ALA4417111 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition in absence of 0.01 % Triton X-100 by continuous spectrophotometric assay | Homo sapiens | 14 | ALA4414654 | assay format | Scientific Literature | |
| 10. | ALA4417112 | B | Binding affinity to human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) at 200 uM in Tris buffer at pH 7.5 in absence of Mg2+ by WaterLOGSY NMR analysis | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 11. | ALA4417113 | B | Binding affinity to human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Homo sapiens | 4 | ALA4414654 | assay format | Scientific Literature | |
| 12. | ALA4417114 | B | Binding affinity to human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as association rate constant by surface plasmon resonance assay | Homo sapiens | 4 | ALA4414654 | assay format | Scientific Literature | |
| 13. | ALA4417115 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) measured after 30 mins | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 14. | ALA4417116 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using IPP as substrate measured after 30 mins | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 15. | ALA4417117 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) using GPP as substrate measured after 30 mins in presence of Mg2+ | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 16. | ALA4417118 | B | Non-competitive inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as substrate Km using varying concentration of GPP as substrate preincubated for 30 mins followed by substrate addition in presence of 35 uM IPP by Michaelis-Menten plot analysis | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 17. | ALA4417119 | B | Non-competitive inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as substrate Km using varying concentration of IPP as substrate preincubated for 30 mins followed by substrate addition in presence of 70 uM GPP by Michaelis-Menten plot analysis | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 18. | ALA4417120 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS expressed in Escherichia coli BL21 (DE3) assessed as enzyme-compound adduct formation at 10 uM measured after 12 hrs by LC-MS analysis | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 19. | ALA4417121 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS N59A mutant expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature | |
| 20. | ALA4417122 | B | Inhibition of human N-terminal TEV protease cleavage site-fused-His6-tagged FPPS F239A mutant expressed in Escherichia coli BL21 (DE3) using GPP and IPP as substrate preincubated for 30 mins followed by substrate addition by continuous spectrophotometric assay | Homo sapiens | 1 | ALA4414654 | assay format | Scientific Literature |
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