# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4433277 | B | Displacement of [3H]-N-alpha-methylhistamine from human recombinant full length H3R expressed in HEK293 cell membranes incubated for 90 mins by liquid scintillation counting method | Homo sapiens | 14 | ALA4433195 | cell-based format | Scientific Literature | |
2. | ALA4433278 | F | Antagonist activity at human H3R expressed in HEK293 cells assessed as reduction in R)(-)-alpha methylhistamine-induced inhibition of forskolin-induced cAMP accumulation by TR-FRET based LANCE ultra cAMP immunoassay | Homo sapiens | 2 | ALA4433195 | cell-based format | Scientific Literature | |
3. | ALA4433279 | F | Reversal of RAMH-induced dipsogenia in Wistar rat (Krf:(WI) WU) assessed as inhibition of RAMH-induced drinking response pretreated for 30 mins followed by RAMH stimulation for 20 mins and measured for 30 mins | Rattus norvegicus | 2 | ALA4433195 | organism-based format | Scientific Literature | |
4. | ALA4433281 | B | Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method | Homo sapiens | 2 | ALA4433195 | cell-based format | Scientific Literature | |
5. | ALA4433282 | B | Antagonist activity at histamine H1 receptor in guinea-pig ileum assessed as inhibition of histamine-induced contractions incubated for 15 mins | Cavia porcellus | 3 | ALA4433195 | tissue-based format | Scientific Literature | |
6. | ALA4433283 | B | Antagonist activity at muscarinic M3 receptor in guinea-pig ileum assessed as inhibition of carbachol-induced contractions incubated for 15 mins | Cavia porcellus | 3 | ALA4433195 | tissue-based format | Scientific Literature | |
7. | ALA4433284 | B | Displacement of [3H]pyrilamine from human H1 receptor expressed in CHOK1 cells incubated for 60 mins by microbeta scintillation counter method | Homo sapiens | 1 | ALA4433195 | cell-based format | Scientific Literature | |
8. | ALA4433285 | B | Inhibition of human ERG expressed in CHO cells at holding potential of -90 mV by patch clamp method | Homo sapiens | 4 | ALA4433195 | cell-based format | Scientific Literature | |
9. | ALA4433286 | B | Selectivity ratio of Ki for human H4R to Ki for human H3R | Homo sapiens | 2 | ALA4433195 | assay format | Scientific Literature | |
10. | ALA4433287 | A | Cytotoxicity against human HepG2 cells assessed as cell viability incubated for 72 hrs by cellTiter 96 AQueous non-radioactive cell proliferation assay | Homo sapiens | 3 | ALA4433195 | cell-based format | Scientific Literature | |
11. | ALA4433288 | A | Ratio of test compound IC50 to doxorubicin IC50 for cytotoxicity against human HepG2 cells assessed as cell viability incubated for 72 hrs by cellTiter 96 AQueous non-radioactive cell proliferation assay | Homo sapiens | 2 | ALA4433195 | cell-based format | Scientific Literature | |
12. | ALA4433289 | T | Mutagenicity in Salmonella typhimurium TA98 assessed as fold increase in number of positive wells over the solvent control baseline upto 10 uM incuabted for 90 mins and measured after 48 hrs by Ames test | Salmonella enterica subsp. enterica serovar Typhimurium | 2 | ALA4433195 | assay format | Scientific Literature | |
13. | ALA4433290 | T | Mutagenicity in Salmonella typhimurium TA100 assessed as fold increase in number of positive wells over the solvent control baseline upto 10 uM incuabted for 90 mins and measured after 48 hrs by Ames test | Salmonella enterica subsp. enterica serovar Typhimurium | 2 | ALA4433195 | assay format | Scientific Literature | |
14. | ALA4433293 | A | Metabolic stability in human recombinant liver microsomes assessed as retention time at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 120 mins by UPLC-MS analysis | Homo sapiens | 6 | ALA4433195 | microsome format | Scientific Literature | |
15. | ALA4433294 | A | Metabolic stability in rat liver microsomes assessed as retention time at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 30 mins UPLC-MS analysis | Rattus norvegicus | 4 | ALA4433195 | microsome format | Scientific Literature | |
16. | ALA4433295 | A | Metabolic stability in human recombinant liver microsomes assessed as metabolite M formation at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 120 mins by UPLC-MS analysis | Homo sapiens | 2 | ALA4433195 | microsome format | Scientific Literature | |
17. | ALA4433296 | A | Metabolic stability in human recombinant liver microsomes assessed as metabolite M1 formation at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 120 mins by UPLC-MS analysis | Homo sapiens | 2 | ALA4433195 | microsome format | Scientific Literature | |
18. | ALA4433297 | A | Metabolic stability in human recombinant liver microsomes assessed as metabolite M2 formation at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 120 mins by UPLC-MS analysis | Homo sapiens | 2 | ALA4433195 | microsome format | Scientific Literature | |
19. | ALA4433298 | A | Metabolic stability in human recombinant liver microsomes assessed as metabolite M3 formation at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 120 mins by UPLC-MS analysis | Homo sapiens | 2 | ALA4433195 | microsome format | Scientific Literature | |
20. | ALA4433299 | A | Metabolic stability in rat liver microsomes assessed as metabolite M1 formation at 50 uM preincubated for 5 mins followed by NADPH addition and measured after 30 mins UPLC-MS analysis | Rattus norvegicus | 1 | ALA4433195 | microsome format | Scientific Literature |