| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4380478 | B | Inhibition of human N-terminal His-tagged MLL1 complex (MLL1/Ash2L/RbBP5/WDR5/DPY-30)-mediated HMT activity using oligonucleosome as substrate preincubated for 15 mins followed by [3H]-SAM addition after 1 hr | Homo sapiens | 21 | ALA4376866 | assay format | Scientific Literature | |
| 2. | ALA4380479 | B | Competitive inhibition of human N-terminal His6-SUMO tagged WDR5 (22 to 334 residues) expressed in Escherichia coli BL21-Gold(DE3) cells using 10-mer-Thr-FAM peptide as substrate after 1 hr by TR-FRET assay | Homo sapiens | 22 | ALA4376866 | cell-based format | Scientific Literature | |
| 3. | ALA4380480 | F | Antiproliferative activity against human MV4-11 cells expressing wild type p53 assessed as growth inhibition after 5 days by CellTiter-Glo luminescent assay | Homo sapiens | 22 | ALA4376866 | cell-based format | Scientific Literature | |
| 4. | ALA4380481 | F | Antiproliferative activity against human MOLM13 cells expressing wild type p53 assessed as growth inhibition after 5 days by CellTiter-Glo luminescent assay | Homo sapiens | 22 | ALA4376866 | cell-based format | Scientific Literature | |
| 5. | ALA4380482 | F | Antiproliferative activity against human K562 cells assessed as growth inhibition after 5 days by CellTiter-Glo luminescent assay | Homo sapiens | 22 | ALA4376866 | cell-based format | Scientific Literature | |
| 6. | ALA4380483 | F | Selectivity index, ratio of GI50 for antiproliferative activity against human K562 cells to GI50 for antiproliferative activity against human MV4-11 cells | Homo sapiens | 22 | ALA4376866 | cell-based format | Scientific Literature | |
| 7. | ALA4380484 | B | Inhibition of WDR5 in human MV4-11 cells assessed as reduction in MLL1 binding to WDR5 WIN site at 1 uM after 4 hrs by co-immunoprecipitation assay | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 8. | ALA4380485 | B | Binding affinity to WDR5 in human MV4-11 cells assessed as WDR5-RBBP5 protein interaction at 1 uM after 4 hrs by co-immunoprecipitation assay | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 9. | ALA4380486 | B | Inhibition of WDR5 in human MV4-11 cells assessed as reduction in WDR5 occupancy at SNHG15 E-box binding site at 600 nM after 4 hrs by chromatin immunoprecipitation assay relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 10. | ALA4380487 | B | Inhibition of WDR5 in human MV4-11 cells assessed as reduction in WDR5 occupancy at RPL35 binding site at 600 nM after 4 hrs by chromatin immunoprecipitation assay relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 11. | ALA4380488 | B | Inhibition of WDR5 in human MV4-11 cells assessed as reduction in WDR5 occupancy at RPS24 binding site at 600 nM after 4 hrs by chromatin immunoprecipitation assay relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 12. | ALA4380489 | B | Inhibition of WDR5 binding to RPS24 in human MV4-11 cells assessed as reduction in RPS24 mRNA expression at 300 nM after 3 days by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 13. | ALA4380490 | B | Inhibition of WDR5 binding to RPS24 in human MV4-11 cells assessed as reduction in RPL35 mRNA expression at 300 nM after 3 days by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 14. | ALA4380491 | A | Effect on RPS11 mRNA expression in human MV4-11 cells at 300 nM after 3 days by qRT-PCR analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 15. | ALA4380492 | A | Effect on RPL14 mRNA expression in human MV4-11 cells at 300 nM after 3 days by qRT-PCR analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 16. | ALA4380493 | B | Inhibition of WDR5 in human MV4-11 cells assessed as increase in p53 protein expression after 24 to 48 hrs by Western blot analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 17. | ALA4380494 | B | Inhibition of WDR5 in human MV4-11 cells assessed as increase in p21 protein expression after 24 to 48 hrs by Western blot analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 18. | ALA4380495 | B | Inhibition of WDR5 in human MV4-11 cells assessed as increase in p53 protein expression at 300 nM after 8 to 32 hrs by Western blot analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 19. | ALA4380496 | B | Inhibition of WDR5 in human MV4-11 cells assessed as increase in p21 protein expression at 300 nM after 8 to 32 hrs by Western blot analysis | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature | |
| 20. | ALA4380497 | F | Induction of apoptosis in human MV4-11 cells assessed as increase in caspase3/7 activity after 48 hrs by caspase-3/7 reagent based luminescence assay relative to control | Homo sapiens | 1 | ALA4376866 | cell-based format | Scientific Literature |
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