# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4372588 | B | Inhibition of deubiquitinase in HEK293T cells expressing N-terminal HA-tagged ubiquitin assessed as stabilization of HMW-Ub proteins after 2 hrs by Western blot analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
2. | ALA4372589 | B | Inhibition of deubiquitinase in COS1 cells expressing N-terminal HA-tagged ubiquitin assessed as stabilization of HMW-Ub proteins by Western blot analysis | Chlorocebus aethiops | 3 | cell-based format | Scientific Literature | ||
3. | ALA4372590 | B | Inhibition of deubiquitinase in HEK293T cells expressing N-terminal HA-tagged ubiquitin assessed as stabilization of HMW-Ub proteins by measuring half life at 250 uM after 2 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
4. | ALA4372591 | B | Inhibition of deubiquitinase in HEK293T cells assessed as stabilization of K48-linked HMW-Ub protein by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
5. | ALA4372619 | B | Inhibition of recombinant His6-tagged USP7 catalytic domain (unknown origin) assessed as association constant using Ub-AMC as substrate by fluorescence assay | Homo sapiens | 11 | single protein format | Scientific Literature | ||
6. | ALA4372620 | B | Inhibition of recombinant His6-tagged USP9x catalytic domain (unknown origin) assessed as association constant using Ub-AMC as substrate by fluorescence assay | Homo sapiens | 9 | single protein format | Scientific Literature | ||
7. | ALA4372621 | B | Inhibition of papaya papain at 100 uM using Z-Arg-AMC as substrate preincubated for 30 mins followed by substrate addition by fluorescence assay | Carica papaya | 5 | single protein format | Scientific Literature | ||
8. | ALA4372622 | B | Inhibition of fig ficin at 100 uM using Z-Arg-AMC as substrate preincubated for 30 mins followed by substrate addition by fluorescence assay | Carica papaya | 5 | assay format | Scientific Literature | ||
9. | ALA4372623 | B | Inhibition of recombinant His6-tagged SENP6 catalytic domain (628 to 1112 residues) (unknown origin) at 1 to 25 uM using SUMO2-modified RanGap1 as substrate preincubated for 30 mins followed by substrate addition and measured after 10 mins by Western blot analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
10. | ALA4372638 | B | Inhibition of 26S proteasome in HEK293T cells expressing GFP-fused Ub G76V mutant assessed as increase in GFP fluorescence | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
11. | ALA4372639 | B | Inhibition of deubiquitinase in human K562 cells assessed as accumulation of K48-linked HMW-Ub protein at 50 uM measured after 2 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
12. | ALA4372640 | B | Inhibition of USP9x in human K562 cells assessed as decrease in Bcr-Abl expression at 50 uM measured after 2 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature |