# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4321228 | Functional | Cytotoxicity against human U266 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay | Homo sapiens | 3 | ALA4316617 | cell-based format | Scientific Literature | |
2. | ALA4321229 | Functional | Cytotoxicity against human MM1S cells assessed as reduction in cell viability measured after 72 hrs by MTT assay | Homo sapiens | 3 | ALA4316617 | cell-based format | Scientific Literature | |
3. | ALA4321230 | Binding | Inhibition of recombinant human Ero1Lalpha C104A/C131A/C166A triple mutant (22 to 468 residues) expressed in Escherichia coli BL21(DE3)-RIL measured after 30 mins by Amplex red dye based fluorescence assay | Homo sapiens | 4 | ALA4316617 | assay format | Scientific Literature | |
4. | ALA4321231 | Binding | Binding affinity to recombinant human His-tagged PDI (18 to 479 residues) expressed in Escherichia coli assessed as melting temperature at 20 uM by SYPRO orange protein gel staining based differential scanning fluorimetry (Rvb = 58.86 +/- 0.12 degreeC) | Homo sapiens | 2 | ALA4316617 | assay format | Scientific Literature | |
5. | ALA4321232 | Binding | Binding affinity to recombinant human Ero1Lalpha C104A/C131A/C166A triple mutant (22 to 468 residues) expressed in Escherichia coli BL21(DE3)-RIL assessed as melting temperature at 20 uM by SYPRO orange protein gel staining based differential scanning fluorimetry (Rvb = 53.15 +/- 0.15 degreeC) | Homo sapiens | 2 | ALA4316617 | assay format | Scientific Literature | |
6. | ALA4321234 | Physicochemical | Solubility of the compound at >200 uM | 2 | ALA4316617 | small-molecule physicochemical format | Scientific Literature |