# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4399624 | Binding | Inhibition of recombinant Staphylococcus aureus wild type truncated C-terminal His6 tagged Srt A at 200 uM using Abz-Leu-Pro-Glu-Thr-Gly-Lys(Dnp)-NH2 as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by FRET assay relative to control | Staphylococcus aureus | 23 | ALA4396929 | single protein format | Scientific Literature | |
2. | ALA4399626 | Binding | Inhibition of recombinant Staphylococcus aureus wild type truncated C-terminal His6 tagged Srt A using Abz-Leu-Pro-Glu-Thr-Gly-Lys(Dnp)-NH2 as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by FRET assay | Staphylococcus aureus | 19 | ALA4396929 | single protein format | Scientific Literature | |
3. | ALA4399632 | Binding | Covalent ligand binding to recombinant Staphylococcus aureus wild type truncated C-terminal His6 tagged SrtA assessed as enzyme modifications by measuring nicotinamido-thiadiazolyl thio (+235.98265 Da, +C8H4N4OS2) and nitrobenzyl-thio (+167.00410, +C7H5NO2S) fragments at 200 uM by LCMS analysis | Staphylococcus aureus | 1 | ALA4396929 | single protein format | Scientific Literature | |
4. | ALA4399634 | Binding | Inhibition of recombinant Staphylococcus aureus wild type truncated C-terminal His6 tagged Srt A using Abz-Leu-Pro-Glu-Thr-Gly-Lys(Dnp)-NH2 as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by FRET assay in presence of 0.5 mM reducing agent, DTT | Staphylococcus aureus | 4 | ALA4396929 | single protein format | Scientific Literature | |
5. | ALA4399638 | Binding | Binding affinity to Staphylococcus aureus SrtA by ligand based 1D CPMG relaxation dispersion NMR analysis | Staphylococcus aureus | 24 | ALA4396929 | single protein format | Scientific Literature |