#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4398579	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin1 neddylation level at 10 uM by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
2.	ALA4398581	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin3 neddylation level by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
3.	ALA4398582	B	Binding affinity to N-terminal His6-tagged recombinant human DCN1 expressed in Escherichia coli BL21-AI assessed as reduction in DCN1-FAM-labelled N-terminal acetylated UBE2M (1 to 12 residues) protein-protein interaction after 30 mins by fluorescence polarization competitive binding assay	Homo sapiens	50		assay format	Scientific Literature
4.	ALA4398583	B	Binding affinity to N-terminal GST tagged recombinant human DCN1 (58 to 259 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1-FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	50		assay format	Scientific Literature
5.	ALA4398584	P	Aqueous solubility of compound in pH 7.4 PBS sonicated for 30 mins followed by 24 hrs incubation under shaking condition by UV-Vis spectroscopy		1		small-molecule physicochemical format	Scientific Literature
6.	ALA4398585	B	Binding affinity to recombinant human DCN1 assessed as thermal stabilization of protein by measuring change in melting temperature at 0.5 mM by SYPRO orange based thermal shift assay	Homo sapiens	3		single protein format	Scientific Literature
7.	ALA4398586	B	Binding affinity to N-terminal GST tagged recombinant DCN2 (unknown origin) (62 to 259 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1-FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
8.	ALA4398587	B	Binding affinity to N-terminal GST tagged recombinant DCN3 (unknown origin) (86 to 304 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
9.	ALA4398588	B	Binding affinity to N-terminal GST tagged recombinant DCN4 (unknown origin) (102 to 292 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
10.	ALA4398589	B	Binding affinity to N-terminal GST tagged recombinant DCN5 (unknown origin) (47 to 237 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
11.	ALA4398590	B	Inhibition of NEDD8-activating enzyme E1C (unknown origin) assessed as effect on NEDD8/UBE2M complex formation up to 100 uM pre-incubated for 10 mins followed by Mg-ATP addition after 60 mins by SDS-PAGE analysis	Homo sapiens	1		single protein format	Scientific Literature
12.	ALA4398592	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as stabilization of DCN1 at 10 uM incubated for 5 hrs at 43 to 61 degC by cellular thermal shift assay	Homo sapiens	2		cell-based format	Scientific Literature
13.	ALA4398593	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as stabilization of DCN1 at 1 uM incubated for 5 hrs at 55 degC by cellular thermal shift assay	Homo sapiens	1		cell-based format	Scientific Literature
14.	ALA4398594	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin2 neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
15.	ALA4398595	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin4A neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
16.	ALA4398596	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin4B neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
17.	ALA4398597	B	Inhibition of DCN1-UBE2M protein-protein interaction in human NCI-H1975 cells assessed as reduction in cullin5 neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
18.	ALA4398598	B	Inhibition of DCN1-UBE2M protein-protein interaction in human PC9 cells assessed as reduction in cullin3 neddylation level by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
19.	ALA4398599	B	Inhibition of DCN1-UBE2M protein-protein interaction in human PC9 cells assessed as reduction in cullin1 neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
20.	ALA4398600	B	Inhibition of DCN1-UBE2M protein-protein interaction in human PC9 cells assessed as reduction in cullin2 neddylation level at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature