# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4411820 | B | Substrate activity at recombinant human NQO1 assessed as ROS generation by measuring metabolic rate utilizing umol of NADPH per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition and measured at 2 secs interval for 5 mins | Homo sapiens | 33 | single protein format | Scientific Literature | ||
2. | ALA4411822 | F | Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
3. | ALA4411823 | F | Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
4. | ALA4411824 | A | Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
5. | ALA4411825 | T | Selectivity ratio of IC50 for NQO1 deficient human L02 cells to IC50 for human A549/TR cells expressing NQO1 | Homo sapiens | 17 | assay format | Scientific Literature | ||
6. | ALA4411912 | B | Substrate activity at recombinant human NQO1 assessed as ratio of number of equivalents of consumed NADPH to number of equivalents of test compound at 10 uM preincubated for 3 mins followed by NADPH addition and measured at 2 secs interval for 5 mins by Michaelis-Menten plot analysis | Homo sapiens | 30 | single protein format | Scientific Literature |