#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4419454	B	Inhibition of ferroportin in mouse J774 cells assessed as reduction in binding of hepcidin to ferroportin pre-incubated for 15 mins before TMR-hepcidin addition and further incubated for 2 hrs by fluorescence based hepcidin internalization assay	Mus musculus	194		cell-based format	Patent Bioactivity Data
2.	ALA4419455	B	Binding affinity to C-terminal FLAG-tagged human ferroportin expressed in Pichia pastoris assessed as reduction in inhibition of binding of TMR-hepcidin to Fpn incubated for 90 mins by fluorescence polarization assay	Homo sapiens	185		assay format	Patent Bioactivity Data
3.	ALA4419456	B	Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as reduction in Fpn-mediated iron efflux incubated overnight by iron regulatory element -driven beta-lactamase reporter gene assay	Homo sapiens	130		cell-based format	Patent Bioactivity Data
4.	ALA4419457	B	Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as induction of ferroportin internalization and degradation incubated overnight by FACS assay	Homo sapiens	107		cell-based format	Patent Bioactivity Data
5.	ALA4419459	B	Inhibition of ferroportin in mouse J774 cells assessed as induce ferroportin ubiquitination and degradation at 1.9 uM incubated for 10 mins by immunoprecipitation assay	Mus musculus	1		cell-based format	Patent Bioactivity Data
6.	ALA4419469	B	Inhibition of ferroportin in mouse J774 cells assessed as induce ferroportin ubiquitination and degradation at 1.9 uM incubated for 120 mins by immunoprecipitation assay	Mus musculus	1		cell-based format	Patent Bioactivity Data
7.	ALA4419480	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 10 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data
8.	ALA4419481	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 30 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data
9.	ALA4419482	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 100 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data
10.	ALA4419484	F	Prevention of iron absorption in anemic Wistar rat model of iron absorption assessed as inhibition of increase in serum iron at 1 mg/kg, po pre-dosed 1 hr before ferrous sulfate dosing and measured 3 hours after iron dosing	Rattus norvegicus	12		organism-based format	Patent Bioactivity Data
11.	ALA4419485	F	Prevention of iron absorption in anemic Wistar rat model of iron absorption assessed as inhibition of increase in serum iron at 3 mg/kg, po pre-dosed 1 hr before ferrous sulfate dosing and measured 3 hours after iron dosing	Rattus norvegicus	12		organism-based format	Patent Bioactivity Data
12.	ALA4419486	F	Prevention of iron absorption in anemic Wistar rat model of iron absorption assessed as inhibition of increase in serum iron at 10 mg/kg, po pre-dosed 1 hr before ferrous sulfate dosing and measured 3 hours after iron dosing	Rattus norvegicus	12		organism-based format	Patent Bioactivity Data
13.	ALA4419487	F	Prevention of iron absorption in anemic Wistar rat model of iron absorption assessed as inhibition of increase in serum iron at 30 mg/kg, po pre-dosed 1 hr before ferrous sulfate dosing and measured 3 hours after iron dosing	Rattus norvegicus	12		organism-based format	Patent Bioactivity Data
14.	ALA4419488	F	Prevention of iron absorption in anemic Wistar rat model of iron absorption assessed as inhibition of increase in serum iron at 100 mg/kg, po pre-dosed 1 hr before ferrous sulfate dosing and measured 3 hours after iron dosing	Rattus norvegicus	12		organism-based format	Patent Bioactivity Data
15.	ALA4419489	F	Prevention of hyperferremia in iron overloaded beta2-microglobulin deficient mouse assessed as reduction in serum iron level at 50 mg/kg, po measured after 3 hrs relative to control	Mus musculus	2		organism-based format	Patent Bioactivity Data
16.	ALA4419494	F	Prevention of iron overload in beta2-microglobulin deficient female mouse assessed as increase in spleen iron accumulation at 20 mg/kg, po BID pre-dosed followed by exposure to iron-containing water and same regime was repeated for 14 days relative to control	Mus musculus	6		organism-based format	Patent Bioactivity Data
17.	ALA4419495	F	Prevention of iron overload in beta2-microglobulin deficient male mouse assessed as increase in spleen iron accumulation at 20 mg/kg, po BID pre-dosed followed by exposure to iron-containing water and same regime was repeated for 14 days relative to control	Mus musculus	6		organism-based format	Patent Bioactivity Data
18.	ALA4419496	F	Prevention of iron overload in beta2-microglobulin deficient female mouse assessed as increase in spleen iron accumulation at 60 mg/kg, po BID pre-dosed followed by exposure to iron-containing water and same regime was repeated for 14 days relative to control	Mus musculus	6		organism-based format	Patent Bioactivity Data
19.	ALA4419497	F	Prevention of iron overload in beta2-microglobulin deficient male mouse assessed as increase in spleen iron accumulation at 60 mg/kg, po BID pre-dosed followed by exposure to iron-containing water and same regime was repeated for 14 days relative to control	Mus musculus	6		organism-based format	Patent Bioactivity Data
20.	ALA4419498	F	Prevention of iron overload in beta2-microglobulin deficient female mouse assessed as reduction in total liver iron accumulation at 20 mg/kg, po BID pre-dosed followed by exposure to iron-containing water and same regime was repeated for 14 days relative to control	Mus musculus	6		organism-based format	Patent Bioactivity Data