Inhibition of glycine transporter-1B in human JAR cells assessed as reduction in [14C]glycine uptake preincubated for 10 mins followed by [14C]glycine addition measured after 3 hrs by scintillation proximity assay
Inhibition of human glycine transporter-2 expressed in HEK293 cells assessed as reduction in [14C]glycine uptake preincubated for 10 mins followed by [14C]glycine addition measured after 3 hrs by scintillation proximity assay
Invivo inhibition of glycine transporter-1 in Sprague-Dawley rat assessed as CSF glycine level at 0.3 mg/kg, po measured 2 hrs post dose by LC-MS/MS analysis relative to control
Invivo inhibition of glycine transporter-1 in Sprague-Dawley rat assessed as CSF glycine level at 1 mg/kg, po measured 2 hrs post dose by LC-MS/MS analysis relative to control
Invivo inhibition of glycine transporter-1 in Sprague-Dawley rat assessed as CSF glycine level at 3 mg/kg, po measured 2 hrs post dose by LC-MS/MS analysis relative to control
Invivo inhibition of glycine transporter-1 in Sprague-Dawley rat assessed as CSF glycine level at 10 mg/kg, po measured 2 hrs post dose by LC-MS/MS analysis relative to control