# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4621971 | Binding | Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli at 1 uM using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis relative to control | Homo sapiens | 11 | ALA4619797 | assay format | Scientific Literature | |
2. | ALA4621972 | Binding | Inhibition of recombinant human nNOS expressed in Escherichia coli at 1 uM using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis relative to control | Homo sapiens | 11 | ALA4619797 | assay format | Scientific Literature | |
3. | ALA4621973 | Binding | Inhibition of human eNOS at 10 uM using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis relative to control | Homo sapiens | 11 | ALA4619797 | single protein format | Scientific Literature | |
4. | ALA4621974 | Binding | Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis | Homo sapiens | 4 | ALA4619797 | assay format | Scientific Literature | |
5. | ALA4621975 | Binding | Inhibition of recombinant human nNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis | Homo sapiens | 2 | ALA4619797 | assay format | Scientific Literature | |
6. | ALA4621976 | ADME | Inhibition of human eNOS using L-argininge as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC analysis | Homo sapiens | 2 | ALA4619797 | single protein format | Scientific Literature | |
7. | ALA4621977 | Binding | Selectivity index, ratio of IC50 for recombinant human nNOS expressed in Escherichia coli to IC50 for recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli | Homo sapiens | 2 | ALA4619797 | assay format | Scientific Literature | |
8. | ALA4621978 | Binding | Selectivity index, ratio of IC50 for recombinant human eNOS to IC50 for recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli | Homo sapiens | 2 | ALA4619797 | assay format | Scientific Literature | |
9. | ALA4621979 | ADME | Cytotoxicity against rat CTX/TNA2 cells assessed as reduction in cell metabolic activity at 1500 uM after 24 hrs by MTT assay | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
10. | ALA4621980 | Functional | Antiproliferative activity against rat C6 cells assessed as reduction in cell metabolic activity after 24 hrs by MTT assay | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
11. | ALA4621981 | Functional | Antiproliferative activity against rat C6 cells assessed as cell metabolic activity at 50 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
12. | ALA4621982 | Functional | Antiproliferative activity against rat C6 cells assessed as cell metabolic activity at 750 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
13. | ALA4621983 | Functional | Antiproliferative activity against rat C6 cells assessed as cell metabolic activity at 1000 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
14. | ALA4621984 | Functional | Antiproliferative activity against rat C6 cells assessed as cell metabolic activity at 1500 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
15. | ALA4621985 | Functional | Antiproliferative activity against rat C6 cells assessed as inhibition of cell proliferation at 500 uM after 24 hrs by MTT assay relative to control | Rattus norvegicus | 2 | ALA4619797 | cell-based format | Scientific Literature | |
16. | ALA4621987 | Functional | Antiproliferative activity against rat C6 cells assessed as increase in LDH release after 24 hrs by CytoTox 96 Non-Radioactive assay | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
17. | ALA4621988 | Functional | Antiproliferative activity against rat C6 cells assessed as LDH release at 250 uM after 24 hrs by CytoTox 96 Non-Radioactive assay (Rvb = 13.49%) | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
18. | ALA4621989 | Functional | Antiproliferative activity against rat C6 cells assessed as LDH release at 500 to 1000 uM after 24 hrs by CytoTox 96 Non-Radioactive assay (Rvb = 13.49%) | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature | |
19. | ALA4621990 | ADME | Cytotoxicity against rat CTX/TNA2 cells assessed as reduction in cell metabolic activity up to 1000 uM after 24 hrs by MTT assay | Rattus norvegicus | 1 | ALA4619797 | cell-based format | Scientific Literature |