Displacement of Alexafluor488 labeled 4-(4-(1-(4-(1-(6-(4-(6-amino-3-imino-4,5-disulfo-3H-xanthen-9-yl)-3-carboxybenzamido)hexyl)-1H-1,2,3-triazol-4-yl)butyl)piperidin-4-yl)phenyl)-7-(4-(trifluoromethyl)phenyl)-2-naphthoic acid from HA-tagged mouse P2Y14 expressed in HEK293 cells measured after 30 mins by FACScalibur flow cytometry analysis
Displacement of Alexafluor488 labeled 4-(4-(1-(4-(1-(6-(4-(6-amino-3-imino-4,5-disulfo-3H-xanthen-9-yl)-3-carboxybenzamido)hexyl)-1H-1,2,3-triazol-4-yl)butyl)piperidin-4-yl)phenyl)-7-(4-(trifluoromethyl)phenyl)-2-naphthoic acid from human P2Y14 expressed in CHO cells measured after 30 mins by FACScalibur flow cytometry analysis
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as reversal of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 30 mins to 3 hrs post-dose by Von Frey assay
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 5 hrs post-dose by Von Frey assay relative to control
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 3 hrs post-dose by Von Frey assay relative to control
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 2 hrs post-dose by Von Frey assay relative to control
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 1 hr post-dose by Von Frey assay relative to control
Antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 0.5 hrs post-dose by Von Frey assay relative to control
Effect on nociception on contralateral paw in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as paw withdrawal threshold at 10 umol/kg, ip administered 7 days post-surgery and measured after 30 mins to 3 hrs post-dose by Von Frey assay
Protection ratio of antinociceptive activity in ICR mouse model of chronic constriction injury-induced neuropathic pain assessed as inhibition of mechanical allodynia at 10 umol/kg, ip administered 7 days post-surgery and measured after 3 hrs to 1 hrs post-dose by Von Frey assay
Inhibition of MRS4174 binding to mouse P2Y14R expressed in HEK293 cells pre-incubated for 30 mins before MRS4174 addition and further incubated for 30 mins by flow cytomtery based competitive fluorescence assay
Selectivity index, ratio of IC50 for inhibition of MRS4174 binding to mouse P2Y14R expressed in HEK292 cells to iC50 for inhibition of MRS4174 binding to human P2Y14R expressed in CHO cells