Inhibition of recombinant human full-length N-terminal GST-tagged p110delta/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells using PIP2 as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by HTRF assay
Selectivity index, ratio IC50 for inhibition of recombinant human full-length N-terminal His6-tagged p110alpha/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells to ratio IC50 for inhibition of recombinant human full-length N-terminal GST-tagged p110delta/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells
Selectivity index, ratio IC50 for inhibition of recombinant human full-length N-terminal His6-tagged p110beta/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells to ratio IC50 for inhibition of recombinant human full-length N-terminal GST-tagged p110delta/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells
Selectivity index, ratio IC50 for inhibition of recombinant human N-terminal His6-tagged full-length p120gamma expressed in Sf21 insect cells to ratio IC50 for inhibition of recombinant human full-length N-terminal GST-tagged p110delta/untagged full-length p85alpha expressed in baculovirus infected Sf21 cells
Inhibition of PI3K delta in human whole blood assessed as inhibition of anti-IgE-stimulated CysLt release in basophils preincubated for 30 mins followed by anti-IgE stimulation and measured after 20 mins