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# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4605951 | Binding | Inhibition of recombinant full length USP7 (unknown origin) using ubiquintin-rhodamine as substrate preincubated for 30 mins followed by substrate addition measured after 1 hr by fluorescence-based Envision plate reader assay | Homo sapiens | 36 | ALA4602719 | single protein format | Scientific Literature | |
2. | ALA4605952 | Binding | Inhibition of His6-tagged human USP1 expressed in insect Sf21 cells coexpressing untagged UAF1 assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 8 | ALA4602719 | cell-based format | Scientific Literature | |
3. | ALA4605953 | Binding | Inhibition of USP47 (unknown origin) by biochemical assay | Homo sapiens | 14 | ALA4602719 | single protein format | Scientific Literature | |
4. | ALA4605954 | Binding | Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measured after 18 hrs by BrightGlo luciferase assay | Homo sapiens | 19 | ALA4602719 | cell-based format | Scientific Literature | |
5. | ALA4605957 | ADME | Cytotoxicity against human RKO cells assessed as reduction in cellular ATP level measured after 5 days by CellTiter-Glo assay | Homo sapiens | 2 | ALA4602719 | cell-based format | Scientific Literature | |
6. | ALA4605958 | Binding | Inhibition of human ERG | Homo sapiens | 4 | ALA4602719 | single protein format | Scientific Literature | |
7. | ALA4605959 | ADME | Inhibition of CYP3A4 (unknown origin) | Homo sapiens | 1 | ALA4602719 | single protein format | Scientific Literature | |
8. | ALA4605962 | ADME | Clearance in Sprague-Dawley rat at 0.5 mg/kg, iv | Rattus norvegicus | 13 | ALA4602719 | organism-based format | Scientific Literature | |
9. | ALA4605963 | ADME | AUC in Sprague-Dawley rat at 2 mg/kg, po | Rattus norvegicus | 13 | ALA4602719 | organism-based format | Scientific Literature | |
10. | ALA4605971 | Binding | Inhibition of human GST-tagged UCHL3 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 3 | ALA4602719 | assay format | Scientific Literature | |
11. | ALA4605972 | Binding | Inhibition of human DAC-tagged USP27X expressed in Escherichia coli assessed as cleavage of KI63-linked di-ubiquitin to mono-ubiquitin using di-ubiquitin as substrate by mass spectrometry | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
12. | ALA4605973 | Binding | Inhibition of human GST-tagged USP2 isoform 4 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
13. | ALA4605974 | Binding | Inhibition of human USP15 isoform 2 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
14. | ALA4605975 | Binding | Inhibition of human 6His-tagged USP4 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
15. | ALA4605976 | Binding | Inhibition of human GST-tagged USP6 CD (529 to 1406 residues) expressed in Sf21 insect cells assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | cell-based format | Scientific Literature | |
16. | ALA4605977 | Binding | Inhibition of human USP8 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
17. | ALA4605978 | Binding | Inhibition of human GST-tagged USP9X CD (1554 to 1995 residues) expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature | |
18. | ALA4605979 | Binding | Inhibition of USP11 (unknown origin) | Homo sapiens | 1 | ALA4602719 | single protein format | Scientific Literature | |
19. | ALA4605980 | Binding | Inhibition of USP16 (unknown origin) | Homo sapiens | 1 | ALA4602719 | single protein format | Scientific Literature | |
20. | ALA4605981 | Binding | Inhibition of human GST-tagged USP19 (1 to 1290 residues) expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | ALA4602719 | assay format | Scientific Literature |