# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4612465 | Binding | Inhibition of SERCA1 in NewZealand rabbit skeletal muscle SR vesicles using ATP as substrate incubated for 2 mins followed by Ca2+ addition by NADH coupled enzyme assay | Oryctolagus cuniculus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
2. | ALA4612466 | Binding | Inhibition of SERCA1 in rat INS-1E cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay | Rattus norvegicus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
3. | ALA4612467 | Binding | Binding affinity to cytosolic region of SERCA1 ATP binding site in NewZealand rabbit SR vesicles assessed as conformational changes by measuring decrease in fluorescence intensity at IC50 incubated for 1 hr by FITC probe labeling based analysis | Oryctolagus cuniculus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
4. | ALA4612468 | Binding | Binding affinity to transmembrane region of SERCA1A ATP binding site in NewZealand rabbit SR vesicles assessed as conformational changes by measuring intrinsic Trp fluorescence at IC50 incubated for 30 mins by spectrofluorometric analysis | Oryctolagus cuniculus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
5. | ALA4612469 | Functional | Induction of SERCA2b expression in rat INS-1E cells at IC50 for cell viability incubated for 24 hrs by Western blot analysis | Rattus norvegicus | 2 | ALA4610111 | cell-based format | Scientific Literature | |
6. | ALA4612470 | Binding | Inhibition of SERCA1 in glucose-stimulated rat INS-1E cells assessed as decline in insulin release preincubated for 24 hrs followed by glucose addition and measured after 30 mins by RIA | Rattus norvegicus | 7 | ALA4610111 | assay format | Scientific Literature | |
7. | ALA4612471 | Binding | Inhibition of SERCA1 in rat INS-1E cells assessed as induction of apoptosis by measuring decrease in living cells at IC50 for cell viability incubated for 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Rattus norvegicus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
8. | ALA4612472 | Binding | Inhibition of SERCA1 in rat INS-1E cells assessed as induction of apoptosis by measuring increase in early apoptotic cells at IC50 for cell viability incubated for 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Rattus norvegicus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
9. | ALA4612473 | Binding | Inhibition of SERCA1 in rat INS-1E cells assessed as induction of apoptosis by measuring increase in late apoptotic cells at IC50 for cell viability incubated for 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Rattus norvegicus | 7 | ALA4610111 | cell-based format | Scientific Literature | |
10. | ALA4612474 | Binding | Inhibition of SERCA1 in rat INS-1E cells assessed as insulin release preincubated for 24 hrs followed by incubation in glucose-free buffer and measured after 30 mins by RIA | Rattus norvegicus | 7 | ALA4610111 | cell-based format | Scientific Literature |