# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4620829 | B | Inhibition of NLRP3 inflammasome activation in LPS-stimulated human THP1 cells assessed as IL-1beta secretion at 10 uM incubated for 1 hr followed by nigericin addition and measured after 1 hr by ELISA relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
2. | ALA4620830 | F | Inhibition of TNF-alpha secretion in LPS-stimulated human THP1 cells assessed as TNF-alpha level at 10 uM incubated for 1 hr followed by nigericin addition and measured after 1 hr by ELISA (Rvb = 957.6 pg/mL) | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
3. | ALA4842435 | B | Inhibition of Yeast GST-tagged/TEV cleavage site fused CRM1 C152/C539 double mutant expressed in Escherichia coli BL21 (DE3) assessed as C1022 conjugation mediated NES inhibition at 60 uM measured after 2 hrs by coomassie blue staining based pull-down assay | Saccharomyces cerevisiae | 1 | assay format | Scientific Literature | ||
4. | ALA4842436 | B | Inhibition of Yeast GST-tagged/TEV cleavage site fused CRM1 C152/C539/D1034A triple mutant expressed in Escherichia coli BL21 (DE3) assessed as decrease in NES binding at 60 uM measured after 2 hrs by coomassie blue staining based pull-down assay | Saccharomyces cerevisiae | 2 | assay format | Scientific Literature | ||
5. | ALA4842437 | B | Inhibition of human wild type CRM1 assessed as C528 conjugation at 20 uM incubated for 2 hrs before binding by coomassie blue staining based pull-down assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
6. | ALA4842438 | B | Inhibition of human CRM1-C528S mutant assessed as C528 conjugation at 20 uM incubated for 2 hrs before binding by coomassie blue staining based pull-down assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
7. | ALA4842439 | B | Inhibition of human CRM1-WT assessed as C528 conjugation at 1 uM incubated for 2 hrs before binding by coomassie blue staining based pull-down assay | Homo sapiens | 1 | single protein format | Scientific Literature |