# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4626912 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT2 expressed in HEK293T cell lysate preincubated for 30 mins followed by MB064 addition and measured after 20 mins by SDS-PAGE based ABPP analysis | Homo sapiens | 56 | ALA4622983 | cell-based format | Scientific Literature | |
2. | ALA4626913 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT3 expressed in HEK293T cell lysate preincubated for 30 mins followed by MB064 addition and measured after 20 mins by SDS-PAGE based ABPP analysis | Homo sapiens | 56 | ALA4622983 | cell-based format | Scientific Literature | |
3. | ALA4626914 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT4 expressed in HEK293T cell lysate preincubated for 30 mins followed by MB064 addition and measured after 20 mins by SDS-PAGE based ABPP analysis | Homo sapiens | 56 | ALA4622983 | cell-based format | Scientific Literature | |
4. | ALA4626915 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT5 expressed in HEK293T cell lysate preincubated for 30 mins followed by MB064 addition and measured after 20 mins by SDS-PAGE based ABPP analysis | Homo sapiens | 56 | ALA4622983 | cell-based format | Scientific Literature | |
5. | ALA4626923 | B | Displacement of [3H]CP55940 from human CB1 receptor expressed in CHOK1 cell membranes at 10 uM by liquid scintillation spectrometry analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
6. | ALA4626924 | B | Displacement of [3H]CP55940 from human CB2 receptor expressed in CHOK1 cell membranes at 10 uM by liquid scintillation spectrometry analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
7. | ALA4626925 | B | Inhibition of full length human NAPE-PLD expressed in HEK293T cells assessed as enzyme remaining activity at 10 uM using PED6 as substrate preincubated for 30 mins followed by substrate addition and measured for 1 hr at 2 mins relative to control | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
8. | ALA4626926 | B | Inhibition of full length human PLA2G4E assessed as enzyme remaining activity at 10 uM using FP-TAMRA as substrate preincubated for 30 mins followed by substrate addition and measured after 5 mins by SDS-PAGE based ABPP analysis relative to control | Homo sapiens | 1 | ALA4622983 | single protein format | Scientific Literature | |
9. | ALA4626927 | B | Inhibition of mouse brain membrane DAGLalpha assessed as enzyme remaining activity at 10 uM preincubated for 30 mins followed by MB064 or FP-TAMRA addition and measured after 15 mins by SDS-PAGE based ABPP analysis relative to control | Mus musculus | 1 | ALA4622983 | cell membrane format | Scientific Literature | |
10. | ALA4626928 | B | Inhibition of mouse brain membrane DAGLbeta assessed as enzyme remaining activity at 10 uM preincubated for 30 mins followed by MB064 or FP-TAMRA addition and measured after 15 mins by SDS-PAGE based ABPP analysis relative to control | Mus musculus | 1 | ALA4622983 | cell membrane format | Scientific Literature | |
11. | ALA4626929 | B | Inhibition of human MAGL expressed in HEK293 cells assessed as enzyme remaining activity at 10 uM preincubated for 1 hr followed by FP-TAMRA addition and measured after 1 hr by SDS-PAGE based ABPP analysis relative to control | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
12. | ALA4626930 | B | Inhibition of mouse brain membrane FAAH assessed as enzyme remaining activity at 10 uM preincubated for 30 mins followed by MB064 or FP-TAMRA addition and measured after 15 mins by SDS-PAGE based ABPP analysis relative to control | Mus musculus | 1 | ALA4622983 | cell membrane format | Scientific Literature | |
13. | ALA4626931 | B | Inhibition of mouse brain membrane ABHD6 assessed as enzyme remaining activity at 10 uM preincubated for 30 mins followed by MB064 or FP-TAMRA addition and measured after 15 mins by SDS-PAGE based ABPP analysis relative to control | Mus musculus | 1 | ALA4622983 | cell membrane format | Scientific Literature | |
14. | ALA4626932 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT2 expressed in human U2OS cells assessed as reduction in anandamide level at 10 uM incubated for 4 hrs by LC-MS/MS analysis relative to control | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
15. | ALA4626933 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT5 expressed in human U2OS cells assessed as reduction in anandamide level at 10 uM incubated for 4 hrs by LC-MS/MS analysis relative to control | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
16. | ALA4626934 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT2 expressed in human U2OS cells assessed as reduction in absolute N-palmitoylethanolamine level at 10 uM incubated for 4 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
17. | ALA4626935 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT5 expressed in human U2OS cells assessed as reduction in absolute N-palmitoylethanolamine level at 10 uM incubated for 4 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
18. | ALA4626936 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT2 expressed in human U2OS cells assessed as reduction in absolute N-palmitoleoylethanolamine level at 10 uM incubated for 4 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
19. | ALA4626937 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT5 expressed in human U2OS cells assessed as reduction in absolute N-palmitoleoylethanolamine level at 10 uM incubated for 4 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature | |
20. | ALA4626938 | B | Inhibition of full-length C-terminal FLAG-tagged human PLAAT2 expressed in human U2OS cells assessed as reduction in absolute N-stearoylethanolamine level at 10 uM incubated for 4 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA4622983 | cell-based format | Scientific Literature |