| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4630260 | B | Inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake at 30 to 300 uM incubated for 15 mins by liquid scintillation counting method | Homo sapiens | 15 | ALA4627406 | cell-based format | Scientific Literature | |
| 2. | ALA4630261 | B | Inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake at 30 uM incubated for 15 mins by liquid scintillation counting method | Homo sapiens | 25 | ALA4627406 | cell-based format | Scientific Literature | |
| 3. | ALA4630262 | B | Ratio of Km for Neu5Ac to Ic50 for test compound for inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake at 30 to 300 uM incubated for 15 mins by liquid scintillation counting method | Homo sapiens | 2 | ALA4627406 | cell-based format | Scientific Literature | |
| 4. | ALA4630263 | B | Inhibition of human LYAAT1 at 30 uM relative to control | Homo sapiens | 1 | ALA4627406 | single protein format | Scientific Literature | |
| 5. | ALA4630264 | B | Inhibition of human cystinosin at 30 uM relative to control | Homo sapiens | 1 | ALA4627406 | single protein format | Scientific Literature | |
| 6. | ALA4630269 | B | Substrate activity mRFP-tagged human recombinant-Sialin L22G/L23G mutant expressed in HEK293 cells assessed as induction of cytosolic acidification by measuring E2GFP fluorescence at 30 uM at pH 5.5 | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 7. | ALA4630273 | B | Non-competitive inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in Vmax for [3H]Neu5Ac uptake at 3 uM incubated for 15 mins by liquid scintillation counting method relative to control | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 8. | ALA4630274 | B | Non-competitive inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake by measuring Km level at 3 uM incubated for 15 mins by liquid scintillation counting method (Rvb = 1.6 +/- 0.3 mM) | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 9. | ALA4630275 | B | Non-competitive inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake incubated for 15 mins by liquid scintillation counting method | Homo sapiens | 2 | ALA4627406 | cell-based format | Scientific Literature | |
| 10. | ALA4630278 | B | Inhibition of human recombinant-Sialin expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac transport at 12 uM pre-incubated for 15 mins at pH 5.5 followed by 15 or 30 mins wash at pH 7 at room temperature by liquid scintillation counting method relative to control | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 11. | ALA4630280 | P | Lipophilicity, log Pof the compound | 3 | ALA4627406 | small-molecule physicochemical format | Scientific Literature | ||
| 12. | ALA4630281 | B | Inhibition of human Sialin R39C mutant expressed in HEK293 cells assessed as reduction in [3H]Neu5Ac uptake at 12 uM incubated for 15 mins by liquid scintillation counting method | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 13. | ALA4630282 | B | Inhibition of cellular distribution of EGFP-tagged human Sialin R39C mutant expressed in human HeLa cells assessed as lysosomal distribution level at 72 uM by LAMP1 immunostaining based fluorescence microscopy | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 14. | ALA4630283 | B | Inhibition of cellular distribution of EGFP-tagged human Sialin R39C mutant expressed in human HeLa cells assessed as non-lysosomal distribution level at 72 uM by LAMP1 immunostaining based fluorescence microscopy | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 15. | ALA4630284 | B | Inhibition of cellular distribution of EGFP-tagged human Sialin expressed in human HeLa cells assessed as lysosomal distribution level at 72 uM by LAMP1 immunostaining based fluorescence microscopy | Homo sapiens | 1 | ALA4627406 | cell-based format | Scientific Literature | |
| 16. | ALA4630285 | B | Inhibition of Sialin R39C mutant in Sall-disease fibroblasts from compound-heterozygote patient assessed as increase in free sialic acid at 30 to 170 uM incubated for 2 days by mass spectrometry | Homo sapiens | 1 | ALA4627406 | assay format | Scientific Literature | |
| 17. | ALA4630286 | B | Inhibition of Sialin R39C/L336W/frameshift alleles in Sall-disease fibroblasts from compound-heterozygote patient assessed as effect on free sialic acid at 30 to 170 uM incubated for 2 days followed by 6 hrs chase in drug free medium by mass spectrometry | Homo sapiens | 1 | ALA4627406 | assay format | Scientific Literature |
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