# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4618409 | Binding | Binding affinity to of proMMP2 in human WM115 cells assessed as reduction in cell membrane association of proMMP at 10 nM incubated for 15 hrs by immunofluorescence based confocal microscopy | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
2. | ALA4618411 | Binding | Binding affinity to fluorescein-labeled D570-A583 epitope of human MMP2 expressed in Escherichia coli BL21(DE3) RIL incubated for 30 mins by competitive FRET assay | Homo sapiens | 1 | ALA4616672 | assay format | Scientific Literature | |
3. | ALA4618413 | Binding | Binding affinity to N-terminal (His6)-MBP tagged full-length human proMMP2 expressed in Escherichia coli BL21(DE3) RIL assessed as reduction in Rhod-cy(WPHPY binding incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 7 | ALA4616672 | assay format | Scientific Literature | |
4. | ALA4618414 | Binding | Binding affinity to hemopexin domain of proMMP2 in human WM115 cells assessed as inhibition of TIMP2-MMP2 interaction using surface immobilized recombinant TIMP incubated for 1 hr by ELISA based competitive binding assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
5. | ALA4618415 | Binding | Inhibition of MMP2 in human WM115 cells at 0.1 nM to 1 uM incubated for 5 mins using MMP2-specific fluorogenic substrate by fluorescence based assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
6. | ALA4618416 | Binding | Binding affinity to catalytic domain of proMMP2 in human WM115 cells assessed as reduction in TIMP2-mediated inhibition of MMP2 enzyme activity at 0.1 nM to 1 uM incubated for 5 mins using MMP2-specific fluorogenic substrate by fluorescence based assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
7. | ALA4618417 | Binding | Inhibition of proMMP2 activation in human WM115 cells assessed as reduction in MMP2 activity at 0.1 nM to 1 uM incubated for 24 hrs by gelatin zymography | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
8. | ALA4618418 | Binding | Inhibition of proMMP2 activation in human WM115 cells assessed as reduction in MMP2 protein expression incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
9. | ALA4618419 | Binding | Inhibition of proMMP2 activation in human WM115 cells assessed as reduction in MMP2 activity at 0.1 nM to 1 uM incubated for 24 hrs using enzyme-specific fluorogenic substrate by fluorescence based assay | Homo sapiens | 2 | ALA4616672 | cell-based format | Scientific Literature | |
10. | ALA4618420 | Binding | Inhibition of proMMP9 activation in human WM115 cells assessed as reduction in MMP9 activity at 0.1 nM to 1 uM incubated for 24 hrs using enzyme-specific fluorogenic substrate by fluorescence based assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
11. | ALA4618421 | Binding | Inhibition of proMMP9 activation in human WM115 cells assessed as decrease in cell numbers in wound region at 1 nM to 1 uM incubated for 24 hrs by wound-healing scratch assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature | |
12. | ALA4618422 | Binding | Inhibition of proMMP9 activation in human WM115 cells assessed as decrease in cell invasion at 1 nM to 1 uM incubated for 24 hrs by transwell cell invasion assay | Homo sapiens | 1 | ALA4616672 | cell-based format | Scientific Literature |