#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4623582	B	Binding affinity to murine eIF4E assessed as intrinsic fluorescence quenching of tryptophan residues by fluorescence spectrophotometric method	Mus musculus	10	ALA4622862	single protein format	Scientific Literature
2.	ALA4623583	B	Displacement of pyrene-labeled 7-methylguanosine-pentaphosphate probe from murine eIF4E assessed as decrease in fluorescence intensity incubated for 15 mins by fluorescence assay	Mus musculus	6	ALA4622862	single protein format	Scientific Literature
3.	ALA4623585	A	Stability of the compound in 10% FBS assessed as compound half life at 50 uM measured up to 4.5 hrs by RP-HPLC analysis	Bos taurus	6	ALA4622862	cell-free format	Scientific Literature
4.	ALA4623586	A	Prodrug activation assessed as recombinant human DcpS mediated compound hydrolysis at 30 uM measured up to 120 mins by RP-HPLC analysis	Homo sapiens	6	ALA4622862	single protein format	Scientific Literature
5.	ALA4623587	A	Prodrug activation assessed as human HINT1 mediated compound hydrolysis at 20 nM measured up to 120 mins by UV-RP-HPLC analysis	Homo sapiens	2	ALA4622862	single protein format	Scientific Literature
6.	ALA4623588	B	Inhibition of human DcpS using pyrene labeled 7-methylguanosine triphosphate as substrate preincubated for 15 mins followed by enzyme addition by fluorescence assay	Homo sapiens	10	ALA4622862	single protein format	Scientific Literature
7.	ALA4623589	A	Prodrug conversion in HEK293 cell extract assessed as m7GMP release at 80 uM measured up to 200 mins by LC-MS/MS analysis	Homo sapiens	5	ALA4622862	cell-based format	Scientific Literature
8.	ALA4623590	A	Permeability of the compound in PBS buffer measured after 16 hrs by PAMPA		5	ALA4622862	assay format	Scientific Literature