# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4628556 | Binding | Induction of CRBN-mediated pLOC-ePL-tagged aiolos (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining up to 10 uM incubated for 4 hrs by luminescence based beta-galactosidase enzyme fragmentation complementation assay relative to control | Homo sapiens | 39 | ALA4627320 | cell-based format | Scientific Literature | |
2. | ALA4628559 | Binding | Displacement of Cy5-conjugated CELMoD from N-terminal 6-His tagged CRBN (1 to 442 residues) (unknown origin)/DDB1 (1 to 1140 residues) (unknown origin) incubated for 10 mins by FRET assay | Homo sapiens | 12 | ALA4627320 | protein format | Scientific Literature | |
3. | ALA4628560 | Physicochemical | Drug metabolism in assay cell medium assessed as compound racemization measured within 24 hrs | 3 | ALA4627320 | small-molecule physicochemical format | Scientific Literature | ||
4. | ALA4628568 | Physicochemical | Drug metabolism in aqueous buffer at pH 7.4 assessed as compound racemization measured within 24 hrs | 2 | ALA4627320 | small-molecule physicochemical format | Scientific Literature | ||
5. | ALA4628569 | Binding | Induction of CRBN-mediated pLOC-ePL-tagged aiolos (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining at 0.00001 to 0.1 uM incubated for 45 to 180 mins by luminescence based beta-galactosidase enzyme fragmentation complementation assay | Homo sapiens | 2 | ALA4627320 | cell-based format | Scientific Literature | |
6. | ALA4628570 | Binding | Induction of CRBN-mediated pLOC-ePL-tagged aiolos (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining at 0.00001 uM incubated for 45 to 180 mins by luminescence based beta-galactosidase enzyme fragmentation complementation assay relative to lenalidomide | Homo sapiens | 1 | ALA4627320 | cell-based format | Scientific Literature | |
7. | ALA4628571 | Binding | Induction of CRBN-mediated pLOC-ePL-tagged aiolos (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining at 0.00001 uM incubated for 45 to 180 mins by luminescence based beta-galactosidase enzyme fragmentation complementation assay relative to pomalidomide | Homo sapiens | 1 | ALA4627320 | cell-based format | Scientific Literature | |
8. | ALA4628572 | Binding | Induction of CRBN-mediated degradation of aiolos in lenalidomide-resistant human H929 R10-1 cells at 1 to 100 nM incubated for 4 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4627320 | cell-based format | Scientific Literature | |
9. | ALA4628573 | Binding | Induction of CRBN-mediated degradation of ikaros in lenalidomide-resistant human H929 R10-1 cells at 1 to 100 nM incubated for 4 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4627320 | cell-based format | Scientific Literature | |
10. | ALA4628574 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as p27 stabilization at 1 to 100 nM after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA4627320 | assay format | Scientific Literature | |
11. | ALA4628575 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as increase in cleaved caspase1 level at 1 to 100 nM after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA4627320 | assay format | Scientific Literature | |
12. | ALA4628576 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as increase in cleaved caspase7 level at 1 to 100 nM after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA4627320 | assay format | Scientific Literature | |
13. | ALA4628577 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as increase in cleaved caspase3 level at 1 to 100 nM after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA4627320 | assay format | Scientific Literature | |
14. | ALA4628578 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as increase in cleaved PARP level after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA4627320 | assay format | Scientific Literature | |
15. | ALA4628579 | Functional | Induction of apoptosis in lenalidomide-resistant human H929-R10-1 cells assessed as caspase3 induction at 1 nM measured over 150 hrs by live cell imaging analysis | Homo sapiens | 1 | ALA4627320 | assay format | Scientific Literature | |
16. | ALA4628581 | ADME | Efflux ratio of permeability across apical to basolateral side in human Caco2 cells | Homo sapiens | 1 | ALA4627320 | cell-based format | Scientific Literature | |
17. | ALA4628582 | Physicochemical | Solubility of the compound at pH 5 | 1 | ALA4627320 | small-molecule physicochemical format | Scientific Literature | ||
18. | ALA4628583 | ADME | Metabolic stability in rat liver S9 fraction assessed as parent compound remaining after 60 mins | Rattus norvegicus | 1 | ALA4627320 | subcellular format | Scientific Literature | |
19. | ALA4628584 | ADME | Metabolic stability in human liver S9 fraction assessed as parent compound remaining after 60 mins | Homo sapiens | 1 | ALA4627320 | subcellular format | Scientific Literature | |
20. | ALA4628585 | ADME | Clearance in Sprague-Dawley rat at 2 mg/kg, iv | Rattus norvegicus | 1 | ALA4627320 | organism-based format | Scientific Literature |