# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4731866 | B | Inhibition of recombinant human ecto-5' nucleotidase expressed in COS7 cell membranes assessed as inorganic phosphate release using AMP as substrate at 100 uM preincubated for 10 mins followed by substrate addition and measured after 20 mins by malachite green dye based spectrophotometry relative to control | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
2. | ALA4731869 | B | Inhibition of recombinant rat ecto-5' nucleotidase expressed in COS7 cell membranes assessed as inorganic phosphate release using AMP as substrate uM preincubated for 10 mins followed by substrate addition and measured after 20 mins by malachite green dye based spectrophotometry | Rattus norvegicus | 8 | cell-based format | Scientific Literature | ||
3. | ALA4731872 | B | Uncompetitive inhibition of rat ecto-5' nucleotidase assessed as increase in Km with constant Vmax at 0 to 0.6 uM by Lineweaver-Burk plot analysis | Rattus norvegicus | 1 | single protein format | Scientific Literature | ||
4. | ALA4731874 | B | Potency index, ratio of sulfamic acid IC50 to compound IC50 for human ecto-5' nucleotidase expressed in COS7 cell membranes assessed as inorganic phosphate release using AMP as substrate preincubated for 10 mins followed by substrate addition and measured after 20 mins by malachite green dye based spectrophotometry | Homo sapiens | 2 | cell-based format | Scientific Literature |