| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4701415 | Binding | Inhibition of His-tagged human CDK9/cyclin T1 expressed in insect cells using biotin Ttds-YISPLKSPYKISEG as substrate preincubated for 15 mins followed by ATP and substrate addition and measured after 25 mins by TR-FRET assay | Homo sapiens | 7 | ALA4699469 | cell-based format | Scientific Literature | |
| 2. | ALA4701416 | Binding | Inhibition of His-tagged human CDK2/cyclin E1 expressed in insect cells using biotin Ttds-YISPLKSPYKISEG as substrate preincubated for 15 mins followed by ATP and substrate addition and measured after 25 mins by TR-FRET assay | Homo sapiens | 4 | ALA4699469 | cell-based format | Scientific Literature | |
| 3. | ALA4701417 | Physicochemical | Thermodynamic solubility of the compound at pH 6.5 incubated for 24 hrs by equilibrium shake flask | 3 | ALA4699469 | small-molecule physicochemical format | Scientific Literature | ||
| 4. | ALA4709751 | Binding | Inhibition of recombinant full length His-tagged human CDK9/cyclin T1 expressed in baculovirus expression system using biotinylated peptide biotin-Ttds-YISPLKSPYKISEG as substrate preincubated for 15 mins followed by ATP and substrate addition and measured after 25 mins by TR-FRET assay | Homo sapiens | 6 | ALA4706546 | assay format | Scientific Literature | |
| 5. | ALA4709754 | Binding | Selectivity ratio of IC50 for GST-tagged human CDK2/GST-tagged cyclin E expressed in sf9 cells to IC50 for recombinant full length His-tagged human CDK9/cyclin T1 expressed in baculovirus expression system | Homo sapiens | 5 | ALA4706546 | cell-based format | Scientific Literature | |
| 6. | ALA4709755 | Functional | Antiproliferative activity against human HeLa cells incubated for 96 hrs by crystal violet staining assay | Homo sapiens | 3 | ALA4706546 | cell-based format | Scientific Literature | |
| 7. | ALA5037432 | Binding | Selectivity ratio of IC50 for inhibition of CDK9 (unknown origin) to IC50 for inhibition of CycT1 (unknown origin) using biotin-Ttds-YISPLKSPYKISE as substrate incubated for 25 min in presence of low ATP by TR-FRET assay | Homo sapiens | 11 | ALA5036331 | assay format | Scientific Literature | |
| 8. | ALA5037433 | Binding | Selectivity ratio of IC50 for inhibition of CDK2 (unknown origin) to IC50 for inhibition of CDK9 (unknown origin) using biotin-Ttds-YISPLKSPYKISE as substrate incubated for 25 min in presence of low ATP by TR-FRET assay | Homo sapiens | 11 | ALA5036331 | assay format | Scientific Literature | |
| 9. | ALA5037434 | Binding | Selectivity ratio of IC50 for inhibition of CDK9 (unknown origin) to IC50 for inhibition of CycT1 (unknown origin) in presence of high ATP incubated for 25 min by TR-FRET assay | Homo sapiens | 19 | ALA5036331 | assay format | Scientific Literature | |
| 10. | ALA5037435 | Binding | Selectivity ratio of IC50 for inhibition of CDK2 (unknown origin) to IC50 for inhibition of CDK9 (unknown origin) in presence of high ATP incubated for 25 min by TR-FRET assay | Homo sapiens | 12 | ALA5036331 | assay format | Scientific Literature | |
| 11. | ALA5037436 | Physicochemical | Aqueous solubility of the compound at pH 6.5 for 24 hrs by HPLC-MS/MS | 11 | ALA5036331 | small-molecule physicochemical format | Scientific Literature | ||
| 12. | ALA5037437 | ADME | Clearance in Wistar-Han rat hepatocytes at 1 uM measured for 2 to 90 mins by LC-MS/MS | Rattus norvegicus | 11 | ALA5036331 | cell-based format | Scientific Literature | |
| 13. | ALA5037438 | Functional | Antiproliferative activity against human A2780 cells assessed as inhibition of cell growth in presence of fetal calf serum incubated for 96 hrs measured by celltiter-glo luminescent cell viability assay | Homo sapiens | 13 | ALA5036331 | cell-based format | Scientific Literature | |
| 14. | ALA5037439 | Functional | Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell growth in presence of fetal calf serum incubated for 96 hrs measured by celltiter-glo luminescent cell viability assay | Homo sapiens | 5 | ALA5036331 | cell-based format | Scientific Literature | |
| 15. | ALA5037440 | Physicochemical | Aqueous solubility of the compound at pH 4 for 24 hrs by HPLC-MS/MS | 4 | ALA5036331 | small-molecule physicochemical format | Scientific Literature | ||
| 16. | ALA5037441 | ADME | Apparent permeability of the compound across apical side to basolateral side in human CaCo-2 cells at 2 uM incubated for 2 hrs | Homo sapiens | 7 | ALA5036331 | cell-based format | Scientific Literature | |
| 17. | ALA5037442 | ADME | Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco2 cells at 2 uM incubated for 2 hrs | Homo sapiens | 6 | ALA5036331 | cell-based format | Scientific Literature | |
| 18. | ALA5037443 | ADME | Clearance in Wistar-Han rat Liver microsomes at 1 uM measured for 2 to 90 mins by LC-MS/MS | Rattus norvegicus | 3 | ALA5036331 | microsome format | Scientific Literature | |
| 19. | ALA5037444 | ADME | Clearance in Wistar-Han rat blood at 0.3 to 0.5 mg/kg, iv measured upto 24 hrs by HPLC with LC-MS/MS | Rattus norvegicus | 4 | ALA5036331 | organism-based format | Scientific Literature | |
| 20. | ALA5037445 | ADME | Volume of distribution at steady state in Wistar-Han rat blood at 0.3 to 0.5 mg/kg, iv measured upto 24 hrs by HPLC with LC-MS/MS | Rattus norvegicus | 4 | ALA5036331 | organism-based format | Scientific Literature |
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