# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4680948 | B | Binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) incubated for 5 mins by PLP fluorescence-based screening assay | Mycobacterium tuberculosis | 15 | assay format | Scientific Literature | ||
2. | ALA4680949 | B | Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as apparent first-order rate constant for the formation of the alpha-aminoacrylate intermediate at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry | Mycobacterium tuberculosis | 19 | assay format | Scientific Literature | ||
3. | ALA5262988 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as amount of S-sulfocysteine produced using O-acetyl-L-serine as substrate incubated for 30 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
4. | ALA5262989 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK1 expressed in Escherichia coli BL21(DE3) assessed as amount of cysteine produced using O-acetyl-L-serine as substrate incubated for 12 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
5. | ALA5262990 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant using PLP as substrate incubated for 5 mins by fluorescence analysis | Mycobacterium tuberculosis | 7 | assay format | Scientific Literature |