# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4681324 | B | Inhibition of GST-tagged human TMPK expressed in Escherichia coli BL21 at 2 uM incubated for 10 mins by luciferase-coupled TMPK assay | Homo sapiens | 12 | ALA4680087 | assay format | Scientific Literature | |
2. | ALA4681325 | B | Binding affinity to GST-tagged human TMPK expressed in Escherichia coli BL21 pre-incubated for 30 mins at 25 degC followed by UV irradiation by HRP based Western blot analysis | Homo sapiens | 1 | ALA4680087 | assay format | Scientific Literature | |
3. | ALA4681326 | B | Binding affinity to GST-tagged human TMPK expressed in Escherichia coli BL21 pre-incubated for 30 mins at 25 degC followed by absence of irradiation by HRP based Western blot analysis | Homo sapiens | 1 | ALA4680087 | assay format | Scientific Literature | |
4. | ALA4681327 | B | Binding affinity to ATP binding site of GST-tagged human TMPK expressed in Escherichia coli BL21 assessed as mass shift of labeled peptides in presence of trypsin by LC-MS/MS analysis | Homo sapiens | 1 | ALA4680087 | assay format | Scientific Literature | |
5. | ALA4681328 | B | Binding affinity to ATP binding site of GST-tagged human TMPK expressed in Escherichia coli BL21 assessed as mass shift of labeled peptides in presence of chymotrypsin by LC-MS/MS analysis | Homo sapiens | 1 | ALA4680087 | assay format | Scientific Literature | |
6. | ALA4681329 | B | Inhibition of tyrosine kinase activity in protein samples from human MDA-MB-231 cells using TK substrate incubated for 30 mins | Homo sapiens | 4 | ALA4680087 | tissue-based format | Scientific Literature |