# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4732437 | ADME | Stability of compound assessed as recombinant human CYP1A1 expressed in baculovirus infected insect cells mediated compound metabolism by measuring parent compound remaining at 1 uM preincubated with NADP+ regenerating system for 10 mins followed by enzyme addition and measured after 60 mins by LC-MS analysis | Homo sapiens | 4 | ALA4732024 | cell-based format | Scientific Literature | |
2. | ALA4732438 | Binding | Inhibition of mTORC2 in human A2058 cells assessed as reduction in PKB phosphorylation at S473 residue incubated for 1 hr by Western blot analysis | Homo sapiens | 9 | ALA4732024 | cell-based format | Scientific Literature | |
3. | ALA4732439 | Binding | Inhibition of mTORC1 in human A2058 cells assessed as reduction in S6 ribosomal protein phosphorylation at Ser235/236 residues incubated for 1 hr by Western blot analysis | Homo sapiens | 9 | ALA4732024 | cell-based format | Scientific Literature | |
4. | ALA4732440 | Binding | Inhibition of alexa fluor 647-labeled kinase tracer 314 binding to recombinant N-terminal His6-tagged p110alpha (unknown origin) by TR-FRET assay | Homo sapiens | 9 | ALA4732024 | single protein format | Scientific Literature | |
5. | ALA4732441 | Binding | Inhibition of alexa fluor 647-labeled kinase tracer 314 binding to recombinant N-terminal GST-fused mTOR (1360 to 2549 residues) (unknown origin) by TR-FRET assay | Homo sapiens | 9 | ALA4732024 | single protein format | Scientific Literature | |
6. | ALA4732442 | Binding | Selectivity index, ratio of Ki for inhibition of alexa fluor 647-labeled kinase tracer 314 binding to recombinant N-terminal His6-tagged p110alpha (unknown origin) to Ki for inhibition of alexa fluor 647-labeled kinase tracer 314 binding to recombinant N-terminal GST-fused mTOR (1360 to 2549 residues) (unknown origin) | Homo sapiens | 9 | ALA4732024 | assay format | Scientific Literature | |
7. | ALA4732443 | ADME | Stability of compound assessed as recombinant human CYP1A2 expressed in baculovirus infected insect cells mediated compound metabolism by measuring parent compound remaining at 1 uM preincubated with NADP+ regenerating system for 10 mins followed by enzyme addition and measured after 60 mins by LC-MS analysis | Homo sapiens | 4 | ALA4732024 | cell-based format | Scientific Literature | |
8. | ALA4732444 | ADME | Substrate activity at human MDR1 expressed in MDCK cells assessed as apparent permeability at 1 uM by UPLS-MS/MS analysis | Homo sapiens | 3 | ALA4732024 | cell-based format | Scientific Literature | |
9. | ALA4732445 | ADME | Substrate activity at human MDR1 expressed in MDCK cells assessed as efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side at 1 uM by UPLS-MS/MS analysis | Homo sapiens | 4 | ALA4732024 | cell-based format | Scientific Literature |