# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4729810 | Binding | Inhibition of recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) using bovine insulin as substrate preincubated for 1 hr followed by substrate addition | Homo sapiens | 108 | ALA4725388 | assay format | Scientific Literature | |
2. | ALA4729814 | Functional | Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay | Homo sapiens | 106 | ALA4725388 | cell-based format | Scientific Literature | |
3. | ALA4729848 | Functional | Upregulation of CALR gene expression in human U-87 MG cells at 20 uM incubated for 4 hrs followed by Bru addition during last 30 mins of incubation by Trizol reagent based gene sequencing analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
4. | ALA4729849 | Functional | Upregulation of HSPA5 gene expression in human U-87 MG cells at 20 uM incubated for 4 hrs followed by Bru addition during last 30 mins of incubation by Trizol reagent based gene sequencing analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
5. | ALA4729850 | Functional | Upregulation of MYZAP gene expression in human U-87 MG cells at 20 uM incubated for 4 hrs followed by Bru addition during last 30 mins of incubation by Trizol reagent based gene sequencing analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
6. | ALA4729851 | Functional | Upregulation of NQO1 gene expression in human U-87 MG cells at 20 uM incubated for 4 hrs followed by Bru addition during last 30 mins of incubation by Trizol reagent based gene sequencing analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
7. | ALA4729852 | Functional | Upregulation of SLC7A11 gene expression in human U-87 MG cells at 20 uM incubated for 4 hrs followed by Bru addition during last 30 mins of incubation by Trizol reagent based gene sequencing analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
8. | ALA4729853 | ADME | Effect on total CALR expression in human U-87 MG cells at 20 uM measured after 12 to 48 hrs by Western blot analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature |