# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4729833 | Binding | Inhibition of recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) at 10 to 40 uM using bovine insulin as substrate preincubated for 1 hr followed by substrate addition | Homo sapiens | 1 | ALA4725388 | assay format | Scientific Literature | |
2. | ALA4729834 | ADME | Binding affinity to fetal bovine serum albumin at 20 uM measured after overnight incubation in presence of cell free DMEM medium by gel based assay | Bos taurus | 2 | ALA4725388 | cell-free format | Scientific Literature | |
3. | ALA4729835 | ADME | Binding affinity to fetal bovine serum albumin at 40 uM measured after 24 hrs in presence of human U-87 MG cells by Western blot analysis | Bos taurus | 1 | ALA4725388 | cell-free format | Scientific Literature | |
4. | ALA4729836 | Binding | Binding affinity to PDIA1 in human U-87 MG cells at 40 uM measured after 24 hrs in presence of fetal bovine serum by Western blot analysis | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
5. | ALA4729837 | Binding | Binding affinity to PDIA1 in human U-87 MG cells at 10 uM measured after 24 hrs in absence of fetal bovine serum by coomassie staining GFP channel based assay | Homo sapiens | 1 | ALA4725388 | cell-based format | Scientific Literature | |
6. | ALA4729844 | Binding | Binding affinity to recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) at 20 uM measured after 24 hrs in presence of BSA by SDS PAGE analysis | Homo sapiens | 2 | ALA4725388 | assay format | Scientific Literature | |
7. | ALA4729845 | ADME | Drug uptake in human A 172 cell cytoplasm at 10 uM measured after 24 hrs by PDI antibody/DAPI staining based confocal microscopic analysis | Homo sapiens | 2 | ALA4725388 | subcellular format | Scientific Literature | |
8. | ALA4729846 | ADME | Drug uptake in human A 172 cell endoplasmic reticulum at 10 uM measured after 24 hrs by PDI antibody/DAPI staining based confocal microscopic analysis | Homo sapiens | 2 | ALA4725388 | subcellular format | Scientific Literature | |
9. | ALA4729847 | ADME | Drug uptake in human A 172 cell nucleus at 10 uM measured after 24 hrs by PDI antibody/DAPI staining based confocal microscopic analysis | Homo sapiens | 2 | ALA4725388 | subcellular format | Scientific Literature |