| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4722525 | ADME | Inhibition of human MetAP1 expressed in Escherichia coli BL21 (DE3) using L-methionine-4-methyl-coumary1-7-amide as substrate preincubated for 30 mins followed by substrate addition and measured at 1 min interval for 30 mins by spectrofluorometric method | Homo sapiens | 16 | ALA4715836 | assay format | Scientific Literature | |
| 2. | ALA4722526 | Binding | Inhibition of Leishmania donovani MetAP1 expressed in Escherichia coli BL21 (DE3) using L-methionine-4-methyl-coumary1-7-amide as substrate preincubated for 30 mins followed by substrate addition and measured at 1 min interval for 30 mins by spectrofluorometric method | Leishmania donovani | 20 | ALA4715836 | assay format | Scientific Literature | |
| 3. | ALA4722528 | Binding | Competitive inhibition of Leishmania donovani MetAP1 expressed in Escherichia coli BL21 (DE3) at 1 to 10 uM using varying levels of L-methionine-4-methyl-coumary1-7-amide as substrate preincubated for 30 mins followed by substrate addition and measured for 30 mins by Lineweaver-burk plot analysis | Leishmania donovani | 3 | ALA4715836 | assay format | Scientific Literature | |
| 4. | ALA4722529 | ADME | Cytotoxicity against mouse embryonic fibroblasts measured after 48 hrs by MTT assay | Mus musculus | 4 | ALA4715836 | tissue-based format | Scientific Literature | |
| 5. | ALA4722530 | Binding | Binding affinity to Leishmania donovani MetAP1 expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Leishmania donovani | 4 | ALA4715836 | assay format | Scientific Literature | |
| 6. | ALA4722531 | ADME | Binding affinity to human MetAP1 expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 4 | ALA4715836 | assay format | Scientific Literature | |
| 7. | ALA4722532 | Binding | Binding affinity to Leishmania donovani MetAP1 expressed in Escherichia coli BL21 (DE3) assessed as intrinsic fluorescence quenching of tryptophan residues measured after 30 mins by fluorescence spectroscopic method | Leishmania donovani | 4 | ALA4715836 | assay format | Scientific Literature |
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