# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4739063 | Binding | Inhibition C-terminal His-tagged KSP motor domain(1 to 369 residues) (unknown origin) expressed in bacteria assessed as inhibition of microtubule-stimulated ATPase activity preincubated for 30 mins followed by addition of ATP and measured after 15 mins by luciferase-derived luminiscence assay | Homo sapiens | 7 | ALA4732246 | assay format | Scientific Literature | |
2. | ALA4739064 | Functional | Cell cycle arrest in human A549 cells assessed as decrease in cell population at G1 phase measured by flow cytometry assay | Homo sapiens | 1 | ALA4732246 | cell-based format | Scientific Literature | |
3. | ALA4739067 | Functional | Cytotoxicity against human A549 cells overexpressing GGT assessed as cell growth inhibition measured after 72 hrs by MTS assay | Homo sapiens | 2 | ALA4732246 | cell-based format | Scientific Literature | |
4. | ALA4739068 | Functional | Cytotoxicity against human NCI-H460 cells expressing low GGT assessed as cell growth inhibition measured after 72 hrs by MTS assay | Homo sapiens | 2 | ALA4732246 | cell-based format | Scientific Literature | |
5. | ALA4739069 | Functional | Cell cycle arrest in human A549 cells assessed as increase in cell population at G2/M phase measured by flow cytometry assay | Homo sapiens | 1 | ALA4732246 | cell-based format | Scientific Literature | |
6. | ALA4739070 | Functional | Cytotoxicity against GGT1-knockdown human A549 cells assessed as cell growth inhibition at 0.03 to 10 uM measured after 72 hrs by MTS assay | Homo sapiens | 1 | ALA4732246 | cell-based format | Scientific Literature | |
7. | ALA4739072 | ADME | Prodrug conversion assessed as bovine kidney GGT-mediated active metabolite,(R)-2-Amino-3-[5-(4-trifluoromethyl-phenyl)-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5-ylsulfanyl]-propionic acid formation at 10 uM incubated for 1 hr by LC-MS analysis | Bos taurus | 1 | ALA4732246 | tissue-based format | Scientific Literature |