Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as apparent first-order rate constant for the formation of the alpha-aminoacrylate intermediate at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry
Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as reduction in amino-acrylate intermediate formation by measuring residual enzyme activity at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry
Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of microbial growth incubated for 5 days by alamar blue assay
Antimycobacterial activity against Mycobacterium tuberculosis H37Rv grown under nutrient starvation condition assessed as reduction in nonreplicating cells count incubated for 7 days at 10 ug/ml
Bactericidal activity against Mycobacterium tuberculosis H37Rv grown under nutrient starvation condition assessed as reduction in nonreplicating cells count incubated for 7 days at 10 ug/ml in presence of 0.2 mM cysteine
Selectivity index, ratio of Kd for binding affinity to Mycobacterium tuberculosis CysK1 to Kd for binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3)
Selectivity index, ratio of Kd for binding affinity to Mycobacterium tuberculosis CysK2 to Kd for binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3)
Selectivity index, ratio of Kd for binding affinity to human CBS to Kd for binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3)
Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK1 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant using PLP as substrate incubated for 5 mins by fluorescence analysis