# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4711105 | Binding | Inhibition of recombinant human ULK1 at 10 uM preincubated for 60 mins followed by substrate addition and measured after 30 mins by ADP-glo assay | Homo sapiens | 28 | ALA4706589 | single protein format | Scientific Literature | |
2. | ALA4711106 | Functional | Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 28 | ALA4706589 | cell-based format | Scientific Literature | |
3. | ALA4711107 | Functional | Antiproliferative activity against human U937 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 28 | ALA4706589 | cell-based format | Scientific Literature | |
4. | ALA4711108 | Functional | Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 28 | ALA4706589 | cell-based format | Scientific Literature | |
5. | ALA4711109 | Functional | Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 28 | ALA4706589 | cell-based format | Scientific Literature | |
6. | ALA4711110 | Functional | Antiproliferative activity against human MCF-7 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 28 | ALA4706589 | cell-based format | Scientific Literature | |
7. | ALA4711111 | Binding | Inhibition of human ULK1 in human A549 cells under normal condition assessed as decrease in phosphorylated-Beclin 1(ser15) level by immunoblotting analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
8. | ALA4711112 | Binding | Inhibition of human ULK1 in human A549 cells under nutrient starvation condition assessed as decrease in phosphorylated-Beclin 1(ser15) level by immunoblotting analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
9. | ALA4711113 | Binding | Inhibition of human ULK1 in human A549 cells under normal condition assessed as decrease in phosphorylated-Atg13 (ser318) level by immunoblotting analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
10. | ALA4711114 | Binding | Inhibition of human ULK1 in human A549 cells under nutrient starvation condition assessed as decrease in phosphorylated- Atg13 (ser318) level by immunoblotting analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
11. | ALA4711115 | Binding | Induction of downregulation of ULK1-mediated autophagy in human A549 cells assessed as phosphorylation at Ser317 preincubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
12. | ALA4711116 | Binding | Inhibition of ULK1 mediated autophagy in human A549 cells assessed as reduction in conversion of LC3 1 to LC3-2 at 2 to 8 uM measured after 1 hrs by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
13. | ALA4711117 | Binding | Inhibition of ULK1 mediated autophagy in human A549 cells assessed as p62 degradation at 2 to 8 uM measured after 1 hrs by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
14. | ALA4711118 | Binding | Inhibition of ULK1 mediated autophagy in human A549 cells assessed as decrease in beclin1 level at 2 to 8 uM measured after 1 hrs by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
15. | ALA4711119 | Binding | Inhibition of ULK1 mediated autophagy in human A549 cells transfected with GFP-mRFP-LC3 assessed as increase in LC3 puncta measured after 6 hrs by fluorescence microscopic analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
16. | ALA4711120 | Functional | Induction of apoptosis in human A549 cells assessed as morphological changes upto 8 uM incubated for 24 hrs in dark by Hoechst 33342 staining based fluorescence microscopy | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
17. | ALA4711121 | Functional | Induction of apoptosis in human A549 cells assessed as reduction in Bcl2 protein expression at 2 to 8 uM by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
18. | ALA4711122 | Functional | Induction of apoptosis in human A549 cells assessed as increase in Bax protein expression at 2 to 8 uM by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature | |
19. | ALA4711123 | Functional | Induction of apoptosis in human A549 cells assessed as increase in caspase 3 protein expression at 2 to 8 uM by Western blot analysis | Homo sapiens | 1 | ALA4706589 | cell-based format | Scientific Literature |