# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4670430 | A | Covalent inhibition of MMP19 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
2. | ALA4670431 | A | Covalent inhibition of MMP14 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
3. | ALA4670432 | A | Covalent inhibition of MMP13 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
4. | ALA4670433 | A | Covalent inhibition of MMP12 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
5. | ALA4670434 | A | Covalent inhibition of MMP9 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
6. | ALA4670435 | A | Covalent inhibition of MMP8 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
7. | ALA4670436 | A | Covalent inhibition of MMP7 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
8. | ALA4670437 | A | Covalent inhibition of MMP3 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
9. | ALA4670438 | A | Covalent inhibition of MMP2 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
10. | ALA4670439 | A | Covalent inhibition of MMP1 (unknown origin) at 25 uM using TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6'-TAMRA)-Ala-Lys-NH2 as substrate preincubated with enzyme for 30 mins followed by substrate addition by fluorescence assay | Homo sapiens | 2 | ALA4665791 | single protein format | Scientific Literature | |
11. | ALA4670440 | A | Cytotoxicity against human iPSC-derived neurons assessed as decrease in SNAP25 signal | Homo sapiens | 7 | ALA4665791 | tissue-based format | Scientific Literature | |
12. | ALA4670441 | A | Cytotoxicity against human iPSC-derived neurons assessed as effect on cell-rounding | Homo sapiens | 7 | ALA4665791 | tissue-based format | Scientific Literature | |
13. | ALA4670443 | A | Cytotoxicity against HEK293 cells assessed inhibition of cell proliferation up to 100 uM after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4665791 | cell-based format | Scientific Literature | |
14. | ALA4670444 | B | Inhibition of Clostridium botulinum BoNT/A intoxicated in hiPSC-derived neurons assessed as reduction in SNAP-25 cleavage using SNAP-25 as substrate treated 30 mins post toxin addition and measured 7 hrs post toxin addition by Western blot analysis | Clostridium botulinum | 6 | ALA4665791 | cell-based format | Scientific Literature | |
15. | ALA4670447 | B | Covalent inhibition of Clostridium botulinum BoNT/A light chain expressed in Escherichia coli BL21 (DE3) assessed as covalent adduct formation by measuring increase in enzyme mass at 100 mM measured after 4 hrs by HR-MS analysis | Clostridium botulinum | 1 | ALA4665791 | assay format | Scientific Literature | |
16. | ALA4670448 | B | Covalent inhibition of Clostridium botulinum BoNT/A light chain C165S mutant expressed in Escherichia coli BL21 (DE3) assessed as covalent adduct formation by measuring increase in enzyme mass at 100 mM measured after 4 hrs by HR-MS analysis | Clostridium botulinum | 1 | ALA4665791 | assay format | Scientific Literature | |
17. | ALA4670449 | B | Time dependent inhibition of Clostridium botulinum BoNT/A light chain C165S mutant expressed in Escherichia coli BL21 (DE3) at 100 uM using SNAPtide flp6 as substrate by fluorescence assay | Clostridium botulinum | 1 | ALA4665791 | assay format | Scientific Literature | |
18. | ALA4670450 | B | Inhibition of Clostridium botulinum BoNT/A C165S light chain mutant expressed in Escherichia coli BL21 (DE3) at 100 uM using SNAPtide flp6 as substrate by fluorescence assay relative to control | Clostridium botulinum | 1 | ALA4665791 | assay format | Scientific Literature | |
19. | ALA4670451 | B | Time dependent inhibition of Clostridium botulinum BoNT/A light chain expressed in Escherichia coli BL21 (DE3) assessed as ratio of Kinact/Ki using SNAPtide flp6 as substrate by measuring fluorescence up to 40 min | Clostridium botulinum | 4 | ALA4665791 | assay format | Scientific Literature | |
20. | ALA4670452 | B | Time dependent inhibition of Clostridium botulinum BoNT/A light chain expressed in Escherichia coli BL21 (DE3) assessed as inactivation rate constant using SNAPtide flp6 as substrate by measuring fluorescence up to 40 min | Clostridium botulinum | 4 | ALA4665791 | assay format | Scientific Literature |