| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4736564 | B | Inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Homo sapiens | 28 | assay format | Scientific Literature | ||
| 2. | ALA4736565 | B | Inhibition of p97 in human HeLa cells assessed as reduction in proteasomal turnover of p97-dependent reporter substrate UbG76V-GFP incubated for 120 mins by luciferase reporter gene assay | Homo sapiens | 28 | cell-based format | Scientific Literature | ||
| 3. | ALA4736566 | F | Inhibition of proteasomal turnover of p97-independent ODD-luciferase reporter substrate in human HeLa cells incubated for 120 mins by luciferase reporter gene assay | Homo sapiens | 28 | cell-based format | Scientific Literature | ||
| 4. | ALA4736567 | U | Selectivity ratio of IC50 for inhibition of proteasomal turnover of p97-independent ODD-luciferase reporter substrate in human HeLa cells to IC50 for inhibition of p97 in human HeLa cells assessed as reduction in proteasomal turnover of p97-dependent reporter substrate UbG76V-GFP | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4736568 | B | Inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins in presence of cysteine by biomol green reagent based assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA4736569 | B | Inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins in presence of DTT by biomol green reagent based assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 7. | ALA4736570 | B | Binding affinity to His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as Cys522-adduct formation by tandem mass spectrometry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA4736571 | B | Inhibition of yeast Cdc48 T532C mutant assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Saccharomyces cerevisiae | 12 | assay format | Scientific Literature | ||
| 9. | ALA4736572 | B | Inhibition of His6-tagged p97 C522A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA4736573 | B | Inhibition of His6-tagged p97 C522T mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4736574 | B | Inhibition of yeast Cdc48 assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Saccharomyces cerevisiae | 1 | assay format | Scientific Literature | ||
| 12. | ALA4736575 | B | Selectivity ratio of IC50 for inhibition of His6-tagged p97 C522A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) to IC50 for inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4736576 | B | Selectivity ratio of IC50 for inhibition of His6-tagged p97 C522T mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) to IC50 for inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA4736577 | B | Selectivity ratio of IC50 for inhibition of yeast Cdc48 T532C mutant to IC50 for inhibition of yeast Cdc48 | Saccharomyces cerevisiae | 1 | assay format | Scientific Literature | ||
| 15. | ALA4736578 | A | Inhibition of hamster NSF assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay | Cricetulus griseus | 1 | single protein format | Scientific Literature | ||
| 16. | ALA4736579 | A | Inhibition of human 19s Rpt3 ATPase activity measured after 60 mins by biomol green reagent based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA4736580 | B | Selectivity ratio of IC50 for hamster NSF to IC50 for His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) | 1 | assay format | Scientific Literature | |||
| 18. | ALA4736581 | A | Inhibition of human 20S proteasome stably expressed in HEK293 cells at 5 to 50 uM using succinyl-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition and measured at 3 mins interval for 30 mins by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA4736582 | B | Irreversible inhibition of p97 in human HeLa cells assessed as reduction in p97-dependent reporter substrate UbG76V-GFP degradation preincubated followed by washout and measured after 17 hrs by luciferase reporter gene assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 20. | ALA4736583 | B | Inhibition of p97 (unknown origin) expressed in HEK293 cells co-expressing TCRalpha-GFP assessed as reduction in ERAD-dependent reporter substrate TCRalpha-GFP degradation at 10 uM incubated for 60 to 120 mins by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
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