#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4715294	B	Protac activity at VHL/IRAK1 in HEK293T cells assessed as induction of IRAK1 degradation at 10 uM measured after 24 hrs by Western blot analysis relative to control	Homo sapiens	8	ALA4706763	cell-based format	Scientific Literature
2.	ALA4715295	B	Protac activity at VHL/IRAK1 in HEK293T cells assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis	Homo sapiens	7	ALA4706763	cell-based format	Scientific Literature
3.	ALA5029478	B	Inhibition of truncated human N-terminal GST-fusion tagged IRAK1 (194 to 712 residues) expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay	Homo sapiens	5	ALA5029152	assay format	Scientific Literature
4.	ALA5029479	B	Inhibition of full length human N-terminal GST-fusion tagged IRAK4 expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay	Homo sapiens	5	ALA5029152	assay format	Scientific Literature
5.	ALA5029480	B	Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 10 uM measured after 24 hrs by Western blot analysis relative to control	Homo sapiens	4	ALA5029152	cell-based format	Scientific Literature
6.	ALA5029481	B	Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis	Homo sapiens	4	ALA5029152	cell-based format	Scientific Literature
7.	ALA5029482	B	Displacement of 5-FAM-labelled DEALA-HypYIPMDDDFQLRSF peptide from VHL (unknown origin) by fluorescence polarization assay	Homo sapiens	4	ALA5029152	single protein format	Scientific Literature
8.	ALA5029485	B	Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 1 uM measured after 24 hrsin presence of proteasome inhibitior MG-132 by Western blot analysis	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
9.	ALA5029486	B	Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 1 uM measured after 24 hrsin presence of VH298 by Western blot analysis	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
10.	ALA5029487	B	Inhibition of wild-type human partial length BIKE (S34 to N329 residues) expressed in bacterial expression system at 1 uM by Kinomescan method	Homo sapiens	1	ALA5029152	assay format	Scientific Literature
11.	ALA5029488	B	Inhibition of wild-type human full length DRAK2 (M1 to C372 residues) expressed in bacterial expression system at 1 uM by Kinomescan method	Homo sapiens	1	ALA5029152	assay format	Scientific Literature
12.	ALA5029489	B	Inhibition of wild-type human partial length YSK4 (T1019 to H1328 residues) expressed in mammalian expression system at 1 uM by Kinomescan method	Homo sapiens	1	ALA5029152	assay format	Scientific Literature
13.	ALA5029490	B	Induction of IRAK1 degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
14.	ALA5029491	B	Induction of GAK degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
15.	ALA5029492	B	Induction of NMRAL1 degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
16.	ALA5029493	B	Inhibition of wild-type human partial length GAK (G13 to Y338 residues) expressed in bacterial expression system at 1 uM by Kinomescan method relative to control	Homo sapiens	1	ALA5029152	assay format	Scientific Literature
17.	ALA5029494	B	Induction of GAK degradation in human Toledo cells at 1 uM measured after 6 hrs by quantitative mass spectrometry relative to control	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature
18.	ALA5029495	B	Protac activity at IRAK1/VHL E3 ligase in human HBL1 assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA5029152	tissue-based format	Scientific Literature
19.	ALA5029496	B	Protac activity at IRAK1/VHL E3 ligase in human OCILY10 assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA5029152	tissue-based format	Scientific Literature
20.	ALA5029497	B	Inhibition of IRAK1 in human HBL1 cells assessed as reduction in phosphorylated IKBA level at up to 1 uM measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA5029152	cell-based format	Scientific Literature