| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4715294 | B | Protac activity at VHL/IRAK1 in HEK293T cells assessed as induction of IRAK1 degradation at 10 uM measured after 24 hrs by Western blot analysis relative to control | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 2. | ALA4715295 | B | Protac activity at VHL/IRAK1 in HEK293T cells assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 3. | ALA5029478 | B | Inhibition of truncated human N-terminal GST-fusion tagged IRAK1 (194 to 712 residues) expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 4. | ALA5029479 | B | Inhibition of full length human N-terminal GST-fusion tagged IRAK4 expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 5. | ALA5029480 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 10 uM measured after 24 hrs by Western blot analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 6. | ALA5029481 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 7. | ALA5029482 | B | Displacement of 5-FAM-labelled DEALA-HypYIPMDDDFQLRSF peptide from VHL (unknown origin) by fluorescence polarization assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 8. | ALA5029485 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 1 uM measured after 24 hrsin presence of proteasome inhibitior MG-132 by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA5029486 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 1 uM measured after 24 hrsin presence of VH298 by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5029487 | B | Inhibition of wild-type human partial length BIKE (S34 to N329 residues) expressed in bacterial expression system at 1 uM by Kinomescan method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA5029488 | B | Inhibition of wild-type human full length DRAK2 (M1 to C372 residues) expressed in bacterial expression system at 1 uM by Kinomescan method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA5029489 | B | Inhibition of wild-type human partial length YSK4 (T1019 to H1328 residues) expressed in mammalian expression system at 1 uM by Kinomescan method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA5029490 | B | Induction of IRAK1 degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA5029491 | B | Induction of GAK degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5029492 | B | Induction of NMRAL1 degradation in human Toledo cells assessed as decrease in protein abundance at 1 uM measured after 6 hrs by quantitative mass spectrometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5029493 | B | Inhibition of wild-type human partial length GAK (G13 to Y338 residues) expressed in bacterial expression system at 1 uM by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA5029494 | B | Induction of GAK degradation in human Toledo cells at 1 uM measured after 6 hrs by quantitative mass spectrometry relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5029495 | B | Protac activity at IRAK1/VHL E3 ligase in human HBL1 assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | tissue-based format | Scientific Literature | ||
| 19. | ALA5029496 | B | Protac activity at IRAK1/VHL E3 ligase in human OCILY10 assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | tissue-based format | Scientific Literature | ||
| 20. | ALA5029497 | B | Inhibition of IRAK1 in human HBL1 cells assessed as reduction in phosphorylated IKBA level at up to 1 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
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