# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4688478 | Binding | Antagonist activity at human TRPM8 transfected in HEK293T cells assessed as reduction in menthol-induced calcium influx measured after 60 mins by Fluo-4 AM dye based fluorescence analysis | Homo sapiens | 22 | ALA4680310 | cell-based format | Scientific Literature | |
2. | ALA4688479 | ADME | Drug metabolism in human liver microsomes assessed as dansyl glutathione adduct formation at 100 microM incubated for 30 mins by HPLC/MS based fluorescence analysis relative to diclofenac dGSH adduct | Homo sapiens | 22 | ALA4680310 | microsome format | Scientific Literature | |
3. | ALA4688480 | ADME | Activation of human PXR ligand binding domain incubated for 1 hr by TR-FRET assay | Homo sapiens | 19 | ALA4680310 | single protein format | Scientific Literature | |
4. | ALA4688481 | ADME | Inhibition of CYP3A4 in human liver microsomes assessed as reduction in 1'-hydroxymidazolam formation using midazolam as substrate at 10 uM preincubated with enzyme and NADPH followed by substrate addition and measured after 10 mins by HPLC/MS-MS analysis relative to control | Homo sapiens | 5 | ALA4680310 | microsome format | Scientific Literature | |
5. | ALA4688482 | ADME | Induction of CYP3A4 mRNA expression in human HepaRG cells assessed as fold increase in mRNA expression at 3 uM incubated for 24 hrs by real time PCR analysis relative to control | Homo sapiens | 5 | ALA4680310 | cell-based format | Scientific Literature | |
6. | ALA4688505 | Toxicity | Toxicity in mouse assessed as convulsions at 300 mg/kg, po | Mus musculus | 2 | ALA4680310 | organism-based format | Scientific Literature |