#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4685843	B	Inhibition of class 1 HDAC in human HeLa cell nuclear extract using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		cell-based format	Scientific Literature
2.	ALA4685844	B	Inhibition of recombinant human N-terminal GST-tagged HDAC7 (518 to end residues) expressed in baculovirus infected Sf9 cells using fluorogenic HDAC 2A substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		cell-based format	Scientific Literature
3.	ALA4685845	B	Inhibition of recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus expression system using Boc-Lys(TFA)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		assay format	Scientific Literature
4.	ALA4685846	B	Inhibition of recombinant human C-terminal His-tagged HDAC5 (656 to 1122 residues) expressed in baculovirus infected Sf9 insect cells using fluorogenic HDAC 2A substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		cell-based format	Scientific Literature
5.	ALA4685847	B	Inhibition of recombinant human C-terminal His-tagged HDAC9 (604 to 1066 residues) expressed in baculovirus infected Sf9 cells using fluorogenic HDAC 2A substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		cell-based format	Scientific Literature
6.	ALA4685848	B	Inhibition of recombinant human full-length N-terminal GST-tagged HDAC6 expressed in baculovirus infected Sf9 insect cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	13		cell-based format	Scientific Literature
7.	ALA4685849	B	Inhibition of amyloid beta (1 to 42) (unknown origin) self aggregation after 24 hrs by thioflavin-T fluorescence method	Homo sapiens	11		single protein format	Scientific Literature
8.	ALA4685850	B	Disaggregation of self-induced amyloid beta (1 to 42) (unknown origin) preformed fibrils at 10 uM measured after 24 hrs by thioflavin-T fluorescence assay relative to control	Homo sapiens	11		single protein format	Scientific Literature
9.	ALA4685851	B	Inhibition of recombinant human C-terminal 6His-tagged AChE (Glu32 to Leu614 residues) expressed in CHO cells using acetylthiocholine iodide as substrate preincubated for 5 mins measured after 15 mins by DTNB reagent based assay	Homo sapiens	10		cell-based format	Scientific Literature
10.	ALA4685852	B	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using fluor de lys SIRT1 as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	3		cell-based format	Scientific Literature
11.	ALA4685853	B	Inhibition of recombinant human C-terminal His-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using fluor de lys SIRT1 as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	3		cell-based format	Scientific Literature
12.	ALA4685854	B	Inhibition of human recombinant C-terminal His-tagged HDAC3 (1 to 428 residues)/N-terminal GST-tagged NCOR2 (395 to 489 residus) expressed in baculovirus infected Sf9 insect cells using fluor de lys SIRT1 as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay	Homo sapiens	3		cell-based format	Scientific Literature
13.	ALA4685855	B	Inhibition of Class 1 HDAC in human Neuro2a cells assessed as increase in acetylated Histone H3 level measured after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
14.	ALA4685856	B	Inhibition of HDAC6 in human Neuro2a cells assessed as increase in alpha-tubulin acetylation measured after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
15.	ALA4685857	A	Cytotoxicity against human Neuro2a cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by celltiter-glo luminescence assay	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4685858	F	Induction of neurite outgrowth in Sprague-Dawley rat primary hippocampal neurons assessed as increase in number of axonal and dendritic neurite incubated for 3 days by DAPI staining based immunofluorescence microscopic analysis	Rattus norvegicus	2		tissue-based format	Scientific Literature
17.	ALA4685860	A	Elimination rate constant in human liver microsomes at 1 uM preincubated for 3 mins followed by NADPH addition and measured up to 60 mins by LC-MS/MS analysis	Homo sapiens	3		microsome format	Scientific Literature
18.	ALA4685861	A	Half life in human liver microsomes at 1 uM preincubated for 3 mins followed by NADPH addition and measured up to 60 mins by LC-MS/MS analysis	Homo sapiens	3		microsome format	Scientific Literature
19.	ALA4685862	A	Intrinsic clearance in human liver microsomes at 1 uM preincubated for 3 mins followed by NADPH addition and measured up to 60 mins by LC-MS/MS analysis	Homo sapiens	3		microsome format	Scientific Literature
20.	ALA4685863	A	Apparent permeability of compound in MDCK-MDR1 cells at 5 uM measured after 2 hrs by LC-MS/MS analysis	Canis lupus familiaris	2		cell-based format	Scientific Literature