# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4710418 | B | Inhibition of Mycobacterium tuberculosis DprE1 expressed in Escherichia coli BL21 (DE3) cells assessed as formation of resorufin using FPR as substrate by Amplex Red hydrogen/peroxidase coupled assay | Mycobacterium tuberculosis | 16 | cell-based format | Scientific Literature | ||
2. | ALA4710419 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Rv ATCC 25618 assessed as inhibition of visible bacterial growth by MABA assay | Mycobacterium tuberculosis | 18 | organism-based format | Scientific Literature | ||
3. | ALA4710420 | P | Half life of the compound in PBS assessed as parent compound remaining by HPLC analysis | 7 | small-molecule physicochemical format | Scientific Literature | |||
4. | ALA4710421 | P | Reactivity of compound towards methyl thioglycolate in PBS measured after 48 hrs by LC/MS/MS | 7 | small-molecule physicochemical format | Scientific Literature | |||
5. | ALA4710425 | B | Covalent binding affinity to Mycobacterium tuberculosis DprE1 assessed as adduct formation by measuring 460 Da shift increase in molecular weight at 100 uM by Mass spectrometry analysis | Mycobacterium tuberculosis | 1 | single protein format | Scientific Literature | ||
6. | ALA4710426 | B | Binding affinity to trypsin digested Mycobacterium tuberculosis DprE1 protein assessed as Cys387 adduct formation with 385-NICVDFPIL-393 sequence at 300 uM by measuring retention time by LC/MS analysis | Mycobacterium tuberculosis | 1 | single protein format | Scientific Literature | ||
7. | ALA4710427 | B | Inhibition of Mycobacterium tuberculosis H37Rv DprE1 assessed as increase in trehalose dimycolates at 100 x MIC incubated for 24 hrs using [14C]acetate by TLC based metabolic labelling assay relative to control | Mycobacterium tuberculosis H37Rv | 6 | single protein format | Scientific Literature | ||
8. | ALA4710428 | B | Inhibition of Mycobacterium tuberculosis H37Rv DprE1 assessed as increase in trehalose monomycolates at 100 x MIC incubated for 24 hrs using [14C]acetate by TLC based metabolic labelling assay relative to control | Mycobacterium tuberculosis H37Rv | 6 | single protein format | Scientific Literature | ||
9. | ALA4710429 | B | Inhibition of Mycobacterium tuberculosis H37Rv DprE1 assessed as increase in trehalose dimycolates at 10 x MIC preincubated for 24 hrs using [14C]acetate by TLC based metabolic labelling assay relative to control | Mycobacterium tuberculosis H37Rv | 5 | single protein format | Scientific Literature | ||
10. | ALA4710430 | B | Inhibition of Mycobacterium tuberculosis H37Rv DprE1 assessed as increase in trehalose monomycolates at 10 x MIC preincubated for 24 hrs using [14C]acetate by TLC based metabolic labelling assay relative to control | Mycobacterium tuberculosis H37Rv | 5 | single protein format | Scientific Literature |