| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4688783 | B | Inhibition of N-terminal His6-tagged recombinant human UCHL5 expressed in Escherichia coli BL21 (DE3) codon cells assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 13 | ALA4680319 | cell-based format | Scientific Literature | |
| 2. | ALA4688785 | B | Inhibition of N-terminal His6-tagged recombinant human UCHL5 co-expressed with N-terminal GST/His6-tagged RPN13 in Escherichia coli BL21 (DE3) codon cells assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate at 100 uM incubated for 30 mins by fluorescence based assay relative to control | Homo sapiens | 1 | ALA4680319 | assay format | Scientific Literature | |
| 3. | ALA4688786 | B | Inhibition of N-terminal His6-tagged recombinant human UCHL5 co-expressed with N-terminal GST/His6-tagged RPN13 in Escherichia coli BL21 (DE3) codon cells assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 1 | ALA4680319 | assay format | Scientific Literature | |
| 4. | ALA4688787 | B | Inhibition of UCHL5 in human MDA-MB-231 cells assessed as reduction in enzyme-Ub-VS conjugation at 25 to 50 uM preincubated for 30 mins followed by incubation with Ub-VS for 11 mins in presence of Ub-VS by Western blot analysis | Homo sapiens | 1 | ALA4680319 | cell-based format | Scientific Literature | |
| 5. | ALA4688788 | B | Inhibition of recombinant human N-terminal MET and His6-tagged UCHL1 (Gln2 to Ala223 residues) expressed in Escherichia coli assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 1 | ALA4680319 | assay format | Scientific Literature | |
| 6. | ALA4688789 | B | Inhibition of recombinant human UCHL3 assessed as reduction in cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ub-Rho 110 as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 1 | ALA4680319 | single protein format | Scientific Literature |
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