# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4681356 | Binding | Allosteric inhibition of C-terminal His-tagged recombinant human HDAC3 expressed in Sf9 cells pre-incubated for 2 hrs before acetylated lysine-aminomethyl coumarin-BOC addition and measured after 2 hrs by fluorescence based assay | Homo sapiens | 30 | ALA4680089 | cell-based format | Scientific Literature | |
2. | ALA4681357 | Binding | Allosteric inhibition of HDAC3 in HEK293 cell lysates pre-incubated for 2 hrs before acetylated lysine-aminomethyl coumarin-BOC addition and measured after 2 hrs by fluorescence based assay | Homo sapiens | 30 | ALA4680089 | subcellular format | Scientific Literature | |
3. | ALA4681367 | Functional | Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability at 250 uM incubated for 48 hrs by Cell-titer-blue cell viability assay | Homo sapiens | 1 | ALA4680089 | cell-based format | Scientific Literature | |
4. | ALA4681374 | Binding | Inhibition of Class 1 histone deacetylase in human MOLM-14 cells assessed as upregulation of acetylated histones H3 levels at 100 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 4 | ALA4680089 | cell-based format | Scientific Literature | |
5. | ALA4681375 | Binding | Inhibition of Class 1 histone deacetylase in human MOLM-14 cells assessed as upregulation of acetylated histones H4 levels at 100 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 4 | ALA4680089 | cell-based format | Scientific Literature |