Antiviral activity against SARS-CoV-2 nCoV-2019 BetaCoV/Wuhan/WIV04/2019 infected in African green monkey VeroE6 cells preincubated for 1 hr followed by viral infection and further replacement of fresh medium containing compound and measured at 24 hrs post infection by qRT-PCR method
Inhibition of SARS-CoV-2 3CLpro preincubated for 30 mins followed by addition of (Dabcyl)KTSAVLQSGFRKM(Glu) peptide substrate and measured after 1.5 hrs by FRET assay
Inhibition of recombinant SARS CoV-2 main protease expressed in Escherichia coli using MCA-AVLQ SGFR-Lys (Dnp)-Lys-NH2 as substrate by FRET based assay
Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in viral infection measured after 24 hrs post infection by plaque reduction assay
Inhibition of N-terminal recombinant SARS CoV 2 3CL protease expressed in Escherichia coli BL21-DE3 cells using MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 as substrate by FRET assay
Inhibition of SARS-CoV-2 3CL protease expressed in Escherichia coli BL21 (DE3) cells assessed as change in relative fluorescence unit using MCA-TSAVLQSGFRK(DNP)M as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate reader based FRET assay
Antiviral activity against SARS-CoV-2 WIV04 infected in African green monkey Vero cells assessed as inhibition of viral proliferation measured at 24 hrs post infection by qRT-PCR analysis
Inhibition of recombinant SARS-CoV-2 Main protease expressed in Escherichia coli using MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured every 30 sec for 10 mins by FRET assay
Antiviral activity against SARS-CoV-2 nCoV-2019BetaCoV/Wuhan/WIV04/2019 infected in African green monkey Vero E6 cells assessed as reduction in viral yield in cell supernatant pretreated with cells for 1 hr followed by viral infection for 2 hrs and replacement with fresh medium containing compounds and measured after 24 hrs by plaque assay
Inhibition of recombinant SARS-CoV-2 Main protease expressed in Escherichia coli using MCA-AVLQSGFR-Lys(DNP)-Lys-NH2 as fluorogenic substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins
Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as inhibition of plaque formation pretreated for 1 hr followed by viral infection and measured 24 hrs post infection